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The end game of chemical genetics: target identification.

Publication ,  Journal Article
Kasper, AC; Baker, JB; Kim, H; Hong, J
Published in: Future medicinal chemistry
July 2009

The use of classical genetic and molecular biology methods along with the sequencing of many genomes has proven crucial for elucidating complex biological processes. Despite being invaluable tools, their limitations have led to a search for more versatile alternatives and, thus, to the use of small molecules. Chemical genetics is a rapidly emerging field that uses small-molecule techniques to probe biological systems and is composed of three parts: natural product or small-molecule libraries, phenotypic screening and target identification. Currently, the biggest hurdle in the overall process of chemical genetics is target identification. Efforts to overcome this obstacle have led to advances in the areas of affinity chromatography, yeast haploinsufficiency, complementary DNA (cDNA) overexpression, DNA microarray, small-molecule microarray and RNA interference (RNAi) technologies. While these technologies continue to undergo further optimization, they have been integral in the identification and/or confirmation of many cellular targets and have seen an increase in applications to the drug-development process.

Duke Scholars

Published In

Future medicinal chemistry

DOI

EISSN

1756-8927

ISSN

1756-8919

Publication Date

July 2009

Volume

1

Issue

4

Start / End Page

727 / 736

Related Subject Headings

  • Small Molecule Libraries
  • Signal Transduction
  • RNA Interference
  • Proteins
  • Microarray Analysis
  • Medicinal & Biomolecular Chemistry
  • Haploinsufficiency
  • Gene Expression Profiling
  • Chromatography, Affinity
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Kasper, A. C., Baker, J. B., Kim, H., & Hong, J. (2009). The end game of chemical genetics: target identification. Future Medicinal Chemistry, 1(4), 727–736. https://doi.org/10.4155/fmc.09.52
Kasper, Amanda C., Joseph B. Baker, Hyoungsu Kim, and Jiyong Hong. “The end game of chemical genetics: target identification.Future Medicinal Chemistry 1, no. 4 (July 2009): 727–36. https://doi.org/10.4155/fmc.09.52.
Kasper AC, Baker JB, Kim H, Hong J. The end game of chemical genetics: target identification. Future medicinal chemistry. 2009 Jul;1(4):727–36.
Kasper, Amanda C., et al. “The end game of chemical genetics: target identification.Future Medicinal Chemistry, vol. 1, no. 4, July 2009, pp. 727–36. Epmc, doi:10.4155/fmc.09.52.
Kasper AC, Baker JB, Kim H, Hong J. The end game of chemical genetics: target identification. Future medicinal chemistry. 2009 Jul;1(4):727–736.
Journal cover image

Published In

Future medicinal chemistry

DOI

EISSN

1756-8927

ISSN

1756-8919

Publication Date

July 2009

Volume

1

Issue

4

Start / End Page

727 / 736

Related Subject Headings

  • Small Molecule Libraries
  • Signal Transduction
  • RNA Interference
  • Proteins
  • Microarray Analysis
  • Medicinal & Biomolecular Chemistry
  • Haploinsufficiency
  • Gene Expression Profiling
  • Chromatography, Affinity
  • 3404 Medicinal and biomolecular chemistry