A single step purification for autolytic zinc proteinases.
Publication
, Journal Article
Wilfong, EM; Locklear, U; Toone, EJ
Published in: Bioorganic & medicinal chemistry letters
January 2010
We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.
Duke Scholars
Published In
Bioorganic & medicinal chemistry letters
DOI
EISSN
1464-3405
ISSN
0960-894X
Publication Date
January 2010
Volume
20
Issue
1
Start / End Page
280 / 282
Related Subject Headings
- Protein Folding
- Oligopeptides
- Medicinal & Biomolecular Chemistry
- Matrix Metalloproteinase 3
- Imidazoles
- Histidine
- Chromatography, Affinity
- Catalytic Domain
- Autolysis
- 3405 Organic chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Wilfong, E. M., Locklear, U., & Toone, E. J. (2010). A single step purification for autolytic zinc proteinases. Bioorganic & Medicinal Chemistry Letters, 20(1), 280–282. https://doi.org/10.1016/j.bmcl.2009.10.114
Wilfong, Erin M., Ursala Locklear, and Eric J. Toone. “A single step purification for autolytic zinc proteinases.” Bioorganic & Medicinal Chemistry Letters 20, no. 1 (January 2010): 280–82. https://doi.org/10.1016/j.bmcl.2009.10.114.
Wilfong EM, Locklear U, Toone EJ. A single step purification for autolytic zinc proteinases. Bioorganic & medicinal chemistry letters. 2010 Jan;20(1):280–2.
Wilfong, Erin M., et al. “A single step purification for autolytic zinc proteinases.” Bioorganic & Medicinal Chemistry Letters, vol. 20, no. 1, Jan. 2010, pp. 280–82. Epmc, doi:10.1016/j.bmcl.2009.10.114.
Wilfong EM, Locklear U, Toone EJ. A single step purification for autolytic zinc proteinases. Bioorganic & medicinal chemistry letters. 2010 Jan;20(1):280–282.
Published In
Bioorganic & medicinal chemistry letters
DOI
EISSN
1464-3405
ISSN
0960-894X
Publication Date
January 2010
Volume
20
Issue
1
Start / End Page
280 / 282
Related Subject Headings
- Protein Folding
- Oligopeptides
- Medicinal & Biomolecular Chemistry
- Matrix Metalloproteinase 3
- Imidazoles
- Histidine
- Chromatography, Affinity
- Catalytic Domain
- Autolysis
- 3405 Organic chemistry