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Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages.

Publication ,  Journal Article
Huang, Y-CT; Li, Z; Carter, JD; Soukup, JM; Schwartz, DA; Yang, IV
Published in: Am J Respir Cell Mol Biol
November 2009

Exposure to pollutant particles increased respiratory morbidity and mortality. The alveolar macrophages (AMs) are one cell type in the lung directly exposed to particles. Upon contact with particles, AMs are activated and produce reactive oxygen species, but the scope of this oxidative stress response remains poorly defined. In this study, we determined the gene expression profile in human AMs exposed to particles, and sought to characterize the global response of pro- and antioxidant genes. We exposed AMs obtained by bronchoscopy from normal individuals to Chapel Hill particulate matter of 2.5-microm diameter or smaller (PM(2.5); 1 microg/ml) or vehicle for 4 hours (n = 6 independent samples). mRNAs were extracted, amplified, and hybridized to Agilent human 1A microarray. Significant genes were identified by significance analysis of microarrays (false discovery rate, 10%; P < or = 0.05) and mapped with Gene Ontology in the Database for Annotation, Visualization, and Integrated Discovery. We found 34 and 41 up- and down-regulated genes, respectively; 22 genes (approximately 30%) were involved in metal binding, and 11 were linked to oxidative stress, including up-regulation of five metallothionein (MT)-1 isoforms. Exogenous MT1 attenuated PM(2.5)-induced H2O2 release. PM(2.5) premixed with MT1 stimulated less H2O2 release. Knockdown of MT1F gene increased PM(2.5)-induced H2O2 release. PM(2.5) at 1 microg/ml did not increase H2O2 release. Mount St. Helens PM(2.5) and acid-extracted Chapel Hill PM(2.5), both poor in metals, did not induce MT1F or H2O2 release. Our results show that PM(2.5) induced a gene expression profile prevalent with genes related to metal binding and oxidative stress in human AMs, independent of oxidative stress. Metals associated with PM may play an important role in particle-induced gene changes.

Duke Scholars

Published In

Am J Respir Cell Mol Biol

DOI

EISSN

1535-4989

Publication Date

November 2009

Volume

41

Issue

5

Start / End Page

544 / 552

Location

United States

Related Subject Headings

  • Up-Regulation
  • Respiratory System
  • RNA Interference
  • Polymerase Chain Reaction
  • Particulate Matter
  • Particle Size
  • Oxidative Stress
  • Oligonucleotide Array Sequence Analysis
  • Metallothionein
  • Macrophages, Alveolar
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, Y.-C., Li, Z., Carter, J. D., Soukup, J. M., Schwartz, D. A., & Yang, I. V. (2009). Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages. Am J Respir Cell Mol Biol, 41(5), 544–552. https://doi.org/10.1165/rcmb.2008-0064OC
Huang, Yuh-Chin T., Zhuowei Li, Jacqueline D. Carter, Joleen M. Soukup, David A. Schwartz, and Ivana V. Yang. “Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages.Am J Respir Cell Mol Biol 41, no. 5 (November 2009): 544–52. https://doi.org/10.1165/rcmb.2008-0064OC.
Huang Y-CT, Li Z, Carter JD, Soukup JM, Schwartz DA, Yang IV. Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages. Am J Respir Cell Mol Biol. 2009 Nov;41(5):544–52.
Huang, Yuh-Chin T., et al. “Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages.Am J Respir Cell Mol Biol, vol. 41, no. 5, Nov. 2009, pp. 544–52. Pubmed, doi:10.1165/rcmb.2008-0064OC.
Huang Y-CT, Li Z, Carter JD, Soukup JM, Schwartz DA, Yang IV. Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages. Am J Respir Cell Mol Biol. 2009 Nov;41(5):544–552.

Published In

Am J Respir Cell Mol Biol

DOI

EISSN

1535-4989

Publication Date

November 2009

Volume

41

Issue

5

Start / End Page

544 / 552

Location

United States

Related Subject Headings

  • Up-Regulation
  • Respiratory System
  • RNA Interference
  • Polymerase Chain Reaction
  • Particulate Matter
  • Particle Size
  • Oxidative Stress
  • Oligonucleotide Array Sequence Analysis
  • Metallothionein
  • Macrophages, Alveolar