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Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma.

Publication ,  Journal Article
Barbas, AS; Downing, TE; Balsara, KR; Tan, HE; Rubinstein, GJ; Holzknecht, ZE; Collins, BH; Parker, W; Davis, RD; Lin, SS
Published in: Eur J Clin Invest
August 2008

BACKGROUND: Chronic aspiration associated with gastro oesophageal reflux disease (GERD) is thought to play a substantial role in the development of asthma, the incidence of which is dramatically increasing in industrially developed countries. The majority of data examining the association between aspiration and asthma has been obtained from epidemiological studies, which show that between 50 and 90% of individuals with asthma experience some element of GERD. This study describes the effect of chronic aspiration on a model of experimentally induced airway hypersensitivity in Balb/c mice. MATERIALS AND METHODS: Four experimental groups were utilized: Aspiration/Asthma, Sham/Asthma, Aspiration/Sham and Sham/Sham. Mice were sensitized with aerosolized 1% ovalbumin on days 1 to 10 (sensitization phase), followed by repeated exposure on days 31 to 40 (challenge phase). Aspiration events occurred on days 1, 8,15, 22, 29, 36, 43 and 50. Animals were sacrificed on days 56 and 57. RESULTS: Chronic aspiration of 10 microL of murine gastric fluid per week for eight weeks produced an injury pattern distinct from that of acute aspiration, with lung injury characterized by hyperplasia, neutrophil infiltration of the bronchioles and relative parenchymal sparing. Aspiration during induction of ovalbumin-induced airway hypersensitivity was associated with a trend toward decreased production of antiovalbumin IgG, antiovalbumin IgE, and total IgE. Further, aspiration induced a substantial and significant increase in antiovalbumin IgG1/IgG2a ratios, consistent with a shift toward a predominantly Th2 response. CONCLUSION: These findings indicate that chronic aspiration has a profound effect on the nature of the immune response to aerosolized allergens in a model of experimentally induced airway hypersensitivity.

Duke Scholars

Published In

Eur J Clin Invest

DOI

EISSN

1365-2362

Publication Date

August 2008

Volume

38

Issue

8

Start / End Page

596 / 602

Location

England

Related Subject Headings

  • Ovalbumin
  • Mice
  • Lung
  • Immunoglobulin E
  • General Clinical Medicine
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Disease Models, Animal
  • Cytokines
  • Bronchoconstrictor Agents
 

Citation

APA
Chicago
ICMJE
MLA
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Barbas, A. S., Downing, T. E., Balsara, K. R., Tan, H. E., Rubinstein, G. J., Holzknecht, Z. E., … Lin, S. S. (2008). Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma. Eur J Clin Invest, 38(8), 596–602. https://doi.org/10.1111/j.1365-2362.2008.01976.x
Barbas, A. S., T. E. Downing, K. R. Balsara, H. E. Tan, G. J. Rubinstein, Z. E. Holzknecht, B. H. Collins, W. Parker, R. D. Davis, and S. S. Lin. “Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma.Eur J Clin Invest 38, no. 8 (August 2008): 596–602. https://doi.org/10.1111/j.1365-2362.2008.01976.x.
Barbas AS, Downing TE, Balsara KR, Tan HE, Rubinstein GJ, Holzknecht ZE, et al. Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma. Eur J Clin Invest. 2008 Aug;38(8):596–602.
Barbas, A. S., et al. “Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma.Eur J Clin Invest, vol. 38, no. 8, Aug. 2008, pp. 596–602. Pubmed, doi:10.1111/j.1365-2362.2008.01976.x.
Barbas AS, Downing TE, Balsara KR, Tan HE, Rubinstein GJ, Holzknecht ZE, Collins BH, Parker W, Davis RD, Lin SS. Chronic aspiration shifts the immune response from Th1 to Th2 in a murine model of asthma. Eur J Clin Invest. 2008 Aug;38(8):596–602.
Journal cover image

Published In

Eur J Clin Invest

DOI

EISSN

1365-2362

Publication Date

August 2008

Volume

38

Issue

8

Start / End Page

596 / 602

Location

England

Related Subject Headings

  • Ovalbumin
  • Mice
  • Lung
  • Immunoglobulin E
  • General Clinical Medicine
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Disease Models, Animal
  • Cytokines
  • Bronchoconstrictor Agents