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Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production.

Publication ,  Journal Article
Laskowitz, DT; Matthew, WD; Bennett, ER; Schmechel, D; Herbstreith, MH; Goel, S; McMillian, MK
Published in: Neuroreport
March 9, 1998
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The human apolipoprotein (apo) E4 isoform is associated with an increased risk for Alzheimer's disease (AD) and poor prognosis after acute CNS injury. Addition of human apoE inhibits murine microglial activation in culture, suggesting that microglia might be an important physiological target of apoE. In the present study, we examined the role of endogenous murine apoE in modulating microglial nitric oxide (NO) production following lipopolysaccharide (LPS) stimulation. Brain cultures from apoE-deficient mouse pups showed enhanced NO production relative to cultures from wild-type mice and from transgenic mice expressing the human apoE3 isoform, demonstrating that endogenous apoE produced by glial cultures is capable of inhibiting microglial function. ApoE produced within the brain may suppress microglial reactivity and thus alter the CNS response to acute and chronic injury.

Duke Scholars

Daniel Todd Laskowitz
Author Daniel Todd Laskowitz Neurology, Neurocritical Care
William Dean Matthew
Author William Dean Matthew Neurology
Ellen Ruth Bennett
Author Ellen Ruth Bennett Neurology, Neurocritical Care
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Published In

Neuroreport

DOI

10.1097/00001756-199803090-00010

ISSN

0959-4965

Publication Date

March 9, 1998

Volume

9

Issue

4

Start / End Page

615 / 618

Location

England

Related Subject Headings

  • Nitric Oxide
  • Neurology & Neurosurgery
  • Microglia
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lipopolysaccharides
  • Humans
  • Cells, Cultured
 

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Laskowitz, D. T., Matthew, W. D., Bennett, E. R., Schmechel, D., Herbstreith, M. H., Goel, S., & McMillian, M. K. (1998). Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production. Neuroreport, 9(4), 615–618. https://doi.org/10.1097/00001756-199803090-00010
Laskowitz, D. T., W. D. Matthew, E. R. Bennett, D. Schmechel, M. H. Herbstreith, S. Goel, and M. K. McMillian. “Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production.” Neuroreport 9, no. 4 (March 9, 1998): 615–18. https://doi.org/10.1097/00001756-199803090-00010.
Laskowitz DT, Matthew WD, Bennett ER, Schmechel D, Herbstreith MH, Goel S, et al. Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production. Neuroreport. 1998 Mar 9;9(4):615–8.
Laskowitz, D. T., et al. “Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production.” Neuroreport, vol. 9, no. 4, Mar. 1998, pp. 615–18. Pubmed, doi:10.1097/00001756-199803090-00010.
Laskowitz DT, Matthew WD, Bennett ER, Schmechel D, Herbstreith MH, Goel S, McMillian MK. Endogenous apolipoprotein E suppresses LPS-stimulated microglial nitric oxide production. Neuroreport. 1998 Mar 9;9(4):615–618.

Published In

Neuroreport

DOI

10.1097/00001756-199803090-00010

ISSN

0959-4965

Publication Date

March 9, 1998

Volume

9

Issue

4

Start / End Page

615 / 618

Location

England

Related Subject Headings

  • Nitric Oxide
  • Neurology & Neurosurgery
  • Microglia
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lipopolysaccharides
  • Humans
  • Cells, Cultured