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Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice.

Publication ,  Journal Article
Xu, PT; Schmechel, D; Rothrock-Christian, T; Burkhart, DS; Qiu, HL; Popko, B; Sullivan, P; Maeda, N; Saunders, AM; Roses, AD; Gilbert, JR
Published in: Neurobiol Dis
1996

Apolipoprotein E (apoE) and its three major alleles (APOE2, E3, and E4) have been implicated in Alzheimer's disease and other neurological disorders. Little is known of the role apoE plays in normal brain function and pathology. To create a model to study apoE in brain, we have generated APOE transgenic mice using microinjection of allele-specific human genomic fragments to establish founders which were then bred to APOE knockout mice lacking a functional mouse apoE protein. This allows the study of apoE without interference from the endogenous mouse APOE gene. Results demonstrate that transgenic lines have been established that transcribe and express apoE appropriately in brain, liver, and other tissues. High cholesterol levels found in APOE knockout mice are substantially corrected in the APOE transgenic lines. ApoE immunoreactivity has been detected in glial cells and selected classes of neurons in all three isoform-specific transgenics. This pattern of immunoreactivity is similar to that observed in nonhuman primates and man, and contrasts with the strictly glial staining pattern of normal rodents.

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Published In

Neurobiol Dis

DOI

ISSN

0969-9961

Publication Date

1996

Volume

3

Issue

3

Start / End Page

229 / 245

Location

United States

Related Subject Headings

  • Transgenes
  • Transcription, Genetic
  • Reference Values
  • Protein Biosynthesis
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Mice, Transgenic
  • Mice
  • Humans
 

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Xu, P. T., Schmechel, D., Rothrock-Christian, T., Burkhart, D. S., Qiu, H. L., Popko, B., … Gilbert, J. R. (1996). Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice. Neurobiol Dis, 3(3), 229–245. https://doi.org/10.1006/nbdi.1996.0023
Xu, P. T., D. Schmechel, T. Rothrock-Christian, D. S. Burkhart, H. L. Qiu, B. Popko, P. Sullivan, et al. “Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice.Neurobiol Dis 3, no. 3 (1996): 229–45. https://doi.org/10.1006/nbdi.1996.0023.
Xu PT, Schmechel D, Rothrock-Christian T, Burkhart DS, Qiu HL, Popko B, Sullivan P, Maeda N, Saunders AM, Roses AD, Gilbert JR. Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice. Neurobiol Dis. 1996;3(3):229–245.
Journal cover image

Published In

Neurobiol Dis

DOI

ISSN

0969-9961

Publication Date

1996

Volume

3

Issue

3

Start / End Page

229 / 245

Location

United States

Related Subject Headings

  • Transgenes
  • Transcription, Genetic
  • Reference Values
  • Protein Biosynthesis
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Mice, Transgenic
  • Mice
  • Humans