Skip to main content

The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background.

Publication ,  Journal Article
Facemire, CS; Griffiths, R; Audoly, LP; Koller, BH; Coffman, TM
Published in: Hypertension
February 2010

Prostaglandin (PG)E(2) has multiple actions that may affect blood pressure. It is synthesized from arachidonic acid by the sequential actions of phospholipases, cyclooxygenases, and PGE synthases. Although microsomal PGE synthase (mPGES)1 is the only genetically verified PGE synthase, results of previous studies examining the consequences of mPGES1 deficiency on blood pressure (BP) are conflicting. To determine whether genetic background modifies the impact of mPGES1 on BP, we generated mPGES1(-/-) mice on 2 distinct inbred backgrounds, DBA/1lacJ and 129/SvEv. On the DBA/1 background, baseline BP was similar between wild-type (WT) and mPGES1(-/-) mice. By contrast, on the 129 background, baseline BPs were significantly higher in mPGES1(-/-) animals than WT controls. During angiotensin II infusion, the DBA/1 mPGES1(-/-) and WT mice developed mild hypertension of similar magnitude, whereas 129-mPGES1(-/-) mice developed more severe hypertension than WT controls. DBA/1 animals developed only minimal albuminuria in response to angiotensin II infusion. By contrast, WT 129 mice had significantly higher levels of albumin excretion than WT DBA/1 and the extent of albuminuria was further augmented in 129 mPGES1(-/-) animals. In WT mice of both strains, the increase in urinary excretion of PGE(2) with angiotensin II was attenuated in mPGES1(-/-) animals. Urinary excretion of thromboxane was unaffected by angiotensin II in the DBA/1 lines but increased more than 4-fold in 129 mPGES1(-/-) mice. These data indicate that genetic background significantly modifies the BP response to mPGES1 deficiency. Exaggerated production of thromboxane may contribute to the robust hypertension and albuminuria in 129 mPGES1-deficient mice.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Hypertension

DOI

EISSN

1524-4563

Publication Date

February 2010

Volume

55

Issue

2

Start / End Page

531 / 538

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Random Allocation
  • RNA, Messenger
  • Prostaglandins
  • Prostaglandin-Endoperoxide Synthases
  • Prostaglandin-E Synthases
  • Probability
  • Microsomes
  • Mice, Knockout
  • Mice, Inbred DBA
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Facemire, C. S., Griffiths, R., Audoly, L. P., Koller, B. H., & Coffman, T. M. (2010). The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background. Hypertension, 55(2), 531–538. https://doi.org/10.1161/HYPERTENSIONAHA.109.145631
Facemire, Carie S., Robert Griffiths, Laurent P. Audoly, Beverly H. Koller, and Thomas M. Coffman. “The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background.Hypertension 55, no. 2 (February 2010): 531–38. https://doi.org/10.1161/HYPERTENSIONAHA.109.145631.
Facemire CS, Griffiths R, Audoly LP, Koller BH, Coffman TM. The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background. Hypertension. 2010 Feb;55(2):531–8.
Facemire, Carie S., et al. “The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background.Hypertension, vol. 55, no. 2, Feb. 2010, pp. 531–38. Pubmed, doi:10.1161/HYPERTENSIONAHA.109.145631.
Facemire CS, Griffiths R, Audoly LP, Koller BH, Coffman TM. The impact of microsomal prostaglandin e synthase 1 on blood pressure is determined by genetic background. Hypertension. 2010 Feb;55(2):531–538.

Published In

Hypertension

DOI

EISSN

1524-4563

Publication Date

February 2010

Volume

55

Issue

2

Start / End Page

531 / 538

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Random Allocation
  • RNA, Messenger
  • Prostaglandins
  • Prostaglandin-Endoperoxide Synthases
  • Prostaglandin-E Synthases
  • Probability
  • Microsomes
  • Mice, Knockout
  • Mice, Inbred DBA