Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5.
Publication
, Journal Article
Clements, DA; Wang, JK; Dionne, CA; Goldfarb, M
Published in: Oncogene
May 1993
We have purified biologically active recombinant human fibroblast growth factor 5 (FGF-5) from Escherichia coli. In the presence of heparin, recombinant FGF-5 is as active as native growth factor, demonstrating that glycosylation does not significantly potentiate FGF-5 activity. FGF-5 can bind and induce autophosphorylation of human FGF receptors (FGFR) 1 and 2. Competition binding studies show that the KD for FGF-5-FGFR-1 and FGF-5-FGFR-2 interactions are both between 0.5 and 1.5 x 10(-9) M.
Duke Scholars
Published In
Oncogene
ISSN
0950-9232
Publication Date
May 1993
Volume
8
Issue
5
Start / End Page
1311 / 1316
Location
England
Related Subject Headings
- Recombinant Proteins
- Receptors, Fibroblast Growth Factor
- Receptors, Cell Surface
- Receptor, Fibroblast Growth Factor, Type 1
- Receptor Protein-Tyrosine Kinases
- Phosphorylation
- Oncology & Carcinogenesis
- Molecular Sequence Data
- Mice
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
Clements, D. A., Wang, J. K., Dionne, C. A., & Goldfarb, M. (1993). Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5. Oncogene, 8(5), 1311–1316.
Clements, D. A., J. K. Wang, C. A. Dionne, and M. Goldfarb. “Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5.” Oncogene 8, no. 5 (May 1993): 1311–16.
Clements DA, Wang JK, Dionne CA, Goldfarb M. Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5. Oncogene. 1993 May;8(5):1311–6.
Clements, D. A., et al. “Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5.” Oncogene, vol. 8, no. 5, May 1993, pp. 1311–16.
Clements DA, Wang JK, Dionne CA, Goldfarb M. Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5. Oncogene. 1993 May;8(5):1311–1316.
Published In
Oncogene
ISSN
0950-9232
Publication Date
May 1993
Volume
8
Issue
5
Start / End Page
1311 / 1316
Location
England
Related Subject Headings
- Recombinant Proteins
- Receptors, Fibroblast Growth Factor
- Receptors, Cell Surface
- Receptor, Fibroblast Growth Factor, Type 1
- Receptor Protein-Tyrosine Kinases
- Phosphorylation
- Oncology & Carcinogenesis
- Molecular Sequence Data
- Mice
- Humans