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Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis.

Publication ,  Journal Article
Sood, AK; Wang, J; Mhawech-Fauceglia, P; Jana, B; Liang, P; Geradts, J
Published in: Cancer Epidemiol Biomarkers Prev
June 2009

We previously described frequent overexpression of Sam-pointed domain containing Ets transcription factor (SPDEF), also known as PDEF, in human breast cancer, and suggested a role for this transcription factor in breast tumor progression. To seek evidence in support of this hypothesis, the MCF-12A breast epithelial cell line was transfected with an SPDEF expression plasmid or with control vector plasmid and the transfected cells tested for their tumorigenic growth in vivo. The data showed that SPDEF expression in MCF-12A cells induced accelerated tumor growth in severe combined immune deficient mice compared with vector-transfected MCF-12A cells. Furthermore, Gene Expression Omnibus and Oncomine databases were mined to determine any correlation between SPDEF expression levels and clinical outcome. High SPDEF expression correlated with poor overall survival of patients with estrogen receptor+ breast cancer, in three independent data sets. In contrast, little correlation was observed between SPDEF expression and cancer relapse or remote metastases. SPDEF expression was further found to be restricted to tumors arising in the luminal epithelial lineage including estrogen receptor+ luminal subtype breast tumors, Her2/neu-positive tumors, and apocrine carcinomas. In contrast, little SPDEF expression was found in the basal subtype of breast tumors. Based on these results, we hypothesize that SPDEF has a function in the specification of the progenitor cells of the luminal epithelial lineage that become targets of oncogenesis in luminal breast cancer.

Duke Scholars

Published In

Cancer Epidemiol Biomarkers Prev

DOI

EISSN

1538-7755

Publication Date

June 2009

Volume

18

Issue

6

Start / End Page

1899 / 1903

Location

United States

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Transfection
  • Survival Analysis
  • Proto-Oncogene Proteins c-ets
  • Mice
  • Humans
  • Female
  • Epidemiology
  • Cell Lineage
  • Cell Line, Tumor
 

Citation

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MLA
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Sood, A. K., Wang, J., Mhawech-Fauceglia, P., Jana, B., Liang, P., & Geradts, J. (2009). Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis. Cancer Epidemiol Biomarkers Prev, 18(6), 1899–1903. https://doi.org/10.1158/1055-9965.EPI-09-0055
Sood, Ashwani K., Jianmin Wang, Paulette Mhawech-Fauceglia, Bagirathan Jana, Ping Liang, and Joseph Geradts. “Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis.Cancer Epidemiol Biomarkers Prev 18, no. 6 (June 2009): 1899–1903. https://doi.org/10.1158/1055-9965.EPI-09-0055.
Sood AK, Wang J, Mhawech-Fauceglia P, Jana B, Liang P, Geradts J. Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis. Cancer Epidemiol Biomarkers Prev. 2009 Jun;18(6):1899–903.
Sood, Ashwani K., et al. “Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis.Cancer Epidemiol Biomarkers Prev, vol. 18, no. 6, June 2009, pp. 1899–903. Pubmed, doi:10.1158/1055-9965.EPI-09-0055.
Sood AK, Wang J, Mhawech-Fauceglia P, Jana B, Liang P, Geradts J. Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis. Cancer Epidemiol Biomarkers Prev. 2009 Jun;18(6):1899–1903.

Published In

Cancer Epidemiol Biomarkers Prev

DOI

EISSN

1538-7755

Publication Date

June 2009

Volume

18

Issue

6

Start / End Page

1899 / 1903

Location

United States

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Transfection
  • Survival Analysis
  • Proto-Oncogene Proteins c-ets
  • Mice
  • Humans
  • Female
  • Epidemiology
  • Cell Lineage
  • Cell Line, Tumor