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Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine.

Publication ,  Journal Article
Lagoo, A; Tseng, CK; Sell, S
Published in: Cytokine
July 1990

Normal peripheral blood B cells produce a soluble factor after activation that is functionally indistinguishable from interleukin 2 (IL 2) and can support B cell proliferation in vitro. Purified rabbit peripheral blood B cells, when stimulated with a combination of ionomycin (0.5 microgram/mL) and phorbol myristate acetate (PMA) (1 ng/mL), secreted a soluble factor in the culture medium that supported the IL 2-dependent cell line CTLL-2. The ability of these supernatants to support CTLL-2 growth was almost completely blocked by rabbit antibodies against human recombinant IL 2 and by the anti-IL 2 receptor monoclonal antibody 7D4. These data strongly suggest that the growth factor secreted by rabbit B cells is IL 2. To examine the possibility that the IL 2 activity detected in the B-cell cultures may be derived from residual T cells, B cells were further purified by successive panning with a pan-T-cell monoclonal antibody, L11-135, and goat anti-rabbit IgG. These highly purified B cells produced levels of IL 2 activity comparable to those produced by the initial B cell populations. Comparison of IL 2 production by decreasing numbers of purified T cells and purified B cells also indicated that the B cells were the source of IL 2 activity. Supernatants of activated B cells could support proliferation of B-cell blasts, and this activity could be completely absorbed by CTLL-2 cells, indicating that IL 2 is a major growth factor for B cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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Published In

Cytokine

DOI

ISSN

1043-4666

Publication Date

July 1990

Volume

2

Issue

4

Start / End Page

272 / 279

Location

England

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Receptors, Interleukin-2
  • Receptors, Antigen, B-Cell
  • Rabbits
  • Lymphokines
  • Lymphocyte Activation
  • Interleukin-2
  • In Vitro Techniques
  • Immunology
 

Citation

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Lagoo, A., Tseng, C. K., & Sell, S. (1990). Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine. Cytokine, 2(4), 272–279. https://doi.org/10.1016/1043-4666(90)90028-r
Lagoo, A., C. K. Tseng, and S. Sell. “Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine.Cytokine 2, no. 4 (July 1990): 272–79. https://doi.org/10.1016/1043-4666(90)90028-r.
Lagoo, A., et al. “Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine.Cytokine, vol. 2, no. 4, July 1990, pp. 272–79. Pubmed, doi:10.1016/1043-4666(90)90028-r.
Journal cover image

Published In

Cytokine

DOI

ISSN

1043-4666

Publication Date

July 1990

Volume

2

Issue

4

Start / End Page

272 / 279

Location

England

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Receptors, Interleukin-2
  • Receptors, Antigen, B-Cell
  • Rabbits
  • Lymphokines
  • Lymphocyte Activation
  • Interleukin-2
  • In Vitro Techniques
  • Immunology