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Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS).

Publication ,  Journal Article
Murdoch, DM; Suchard, MS; Venter, WDF; Mhlangu, P; Ottinger, JS; Feldman, C; Van Rie, A; Glencross, DK; Stevens, WS; Weinhold, KJ
Published in: AIDS Res Ther
July 16, 2009

OBJECTIVE: To immunophenotype CD4+ and CD8+ T cell sub-populations in HIV-associated immune reconstitution inflammatory syndrome (IRIS). DESIGN: Nested case-control immunological study. METHODS: ART-naïve HIV-infected patients were prospectively observed for IRIS during the first 6 months of ART. Twenty-two IRIS cases and 22 ART-duration matched controls were sampled for T cell immunophenotyping. RESULTS: IRIS cases demonstrated significantly lower CD4 cell counts compared to controls (baseline: 79 versus 142, p = 0.02; enrollment: 183 versus 263, p = 0.05, respectively) with no differences in HIV RNA levels. Within CD4+T cells, cases exhibited more of an effector memory phenotype compared to controls (40.8 versus 27.0%, p = 0.20), while controls trended towards a central memory phenotype (43.8 versus 30.8%, p = 0.07). Within CD8+ T cells, controls exhibited more central memory (13.9 versus 7.81%, p = 0.01, respectively) and effector (13.2 versus 8.8%, p = 0.04, respectively) phenotypes compared to cases, whereas cases demonstrated more terminal effectors than controls (28.8 versus 15.1%, p = 0.05). Cases demonstrated increased activation of CD8+ T cell effector memory, terminal effector, and effector subsets than controls (p = 0.04, 0.02, and 0.02, respectively). CONCLUSION: CD4+ and CD8+ T cell subset maturational phenotypes were heterogeneous among IRIS cases and controls. However, IRIS cases demonstrated significant increases in activation of CD8+ T cell effector subpopulations.

Duke Scholars

Published In

AIDS Res Ther

DOI

EISSN

1742-6405

Publication Date

July 16, 2009

Volume

6

Start / End Page

16

Location

England

Related Subject Headings

  • Virology
  • 3202 Clinical sciences
  • 1107 Immunology
 

Citation

APA
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MLA
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Murdoch, D. M., Suchard, M. S., Venter, W. D. F., Mhlangu, P., Ottinger, J. S., Feldman, C., … Weinhold, K. J. (2009). Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS). AIDS Res Ther, 6, 16. https://doi.org/10.1186/1742-6405-6-16
Murdoch, David M., Melinda S. Suchard, Willem D. F. Venter, Patrick Mhlangu, Janet S. Ottinger, Charles Feldman, Annelies Van Rie, Deborah K. Glencross, Wendy S. Stevens, and Kent J. Weinhold. “Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS).AIDS Res Ther 6 (July 16, 2009): 16. https://doi.org/10.1186/1742-6405-6-16.
Murdoch DM, Suchard MS, Venter WDF, Mhlangu P, Ottinger JS, Feldman C, et al. Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS). AIDS Res Ther. 2009 Jul 16;6:16.
Murdoch, David M., et al. “Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS).AIDS Res Ther, vol. 6, July 2009, p. 16. Pubmed, doi:10.1186/1742-6405-6-16.
Murdoch DM, Suchard MS, Venter WDF, Mhlangu P, Ottinger JS, Feldman C, Van Rie A, Glencross DK, Stevens WS, Weinhold KJ. Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS). AIDS Res Ther. 2009 Jul 16;6:16.
Journal cover image

Published In

AIDS Res Ther

DOI

EISSN

1742-6405

Publication Date

July 16, 2009

Volume

6

Start / End Page

16

Location

England

Related Subject Headings

  • Virology
  • 3202 Clinical sciences
  • 1107 Immunology