Skip to main content
Journal cover image

Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer.

Publication ,  Journal Article
Dollins, CM; Nair, S; Boczkowski, D; Lee, J; Layzer, JM; Gilboa, E; Sullenger, BA
Published in: Chem Biol
July 21, 2008

We show that a molecular scaffold can be utilized to convert a receptor binding aptamer into a receptor agonist. Many receptors (including tumor necrosis receptor family members) are activated when they are multimerized on the cell surface. Molecular scaffolds have been utilized to assemble multiple receptor binding peptide ligands to generate activators of such receptors. We demonstrate that an RNA aptamer that recognizes OX40, a member of the tumor necrosis factor receptor superfamily, can be converted into a receptor-activating aptamer by assembling two copies on an olignucleotide-based scaffold. The OX40 receptor-activating aptamer is able to induce nuclear localization of nuclear factor-kappaB, cytokine production, and cell proliferation, as well as enhance the potency of dendritic cell-based tumor vaccines when systemically delivered to mice.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Chem Biol

DOI

ISSN

1074-5521

Publication Date

July 21, 2008

Volume

15

Issue

7

Start / End Page

675 / 682

Location

United States

Related Subject Headings

  • Technology, Pharmaceutical
  • Receptors, OX40
  • Organic Chemistry
  • Neoplasm Transplantation
  • NF-kappa B
  • Mice, Inbred C57BL
  • Mice
  • Ligands
  • Female
  • Drug Design
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dollins, C. M., Nair, S., Boczkowski, D., Lee, J., Layzer, J. M., Gilboa, E., & Sullenger, B. A. (2008). Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer. Chem Biol, 15(7), 675–682. https://doi.org/10.1016/j.chembiol.2008.05.016
Dollins, Claudia M., Smita Nair, David Boczkowski, Jaewoo Lee, Juliana M. Layzer, Eli Gilboa, and Bruce A. Sullenger. “Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer.Chem Biol 15, no. 7 (July 21, 2008): 675–82. https://doi.org/10.1016/j.chembiol.2008.05.016.
Dollins CM, Nair S, Boczkowski D, Lee J, Layzer JM, Gilboa E, et al. Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer. Chem Biol. 2008 Jul 21;15(7):675–82.
Dollins, Claudia M., et al. “Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer.Chem Biol, vol. 15, no. 7, July 2008, pp. 675–82. Pubmed, doi:10.1016/j.chembiol.2008.05.016.
Dollins CM, Nair S, Boczkowski D, Lee J, Layzer JM, Gilboa E, Sullenger BA. Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer. Chem Biol. 2008 Jul 21;15(7):675–682.
Journal cover image

Published In

Chem Biol

DOI

ISSN

1074-5521

Publication Date

July 21, 2008

Volume

15

Issue

7

Start / End Page

675 / 682

Location

United States

Related Subject Headings

  • Technology, Pharmaceutical
  • Receptors, OX40
  • Organic Chemistry
  • Neoplasm Transplantation
  • NF-kappa B
  • Mice, Inbred C57BL
  • Mice
  • Ligands
  • Female
  • Drug Design