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Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells.

Publication ,  Journal Article
Devi, GR
Published in: Methods Mol Biol
2009

Phosphorodiamidate morpholino oligomers (PMO), which have a neutral chemistry, are extensively being used as tools for selective inhibition of gene expression in cell culture models and are currently in human clinical trials. PMO oligomers possess a unique structure, in which the deoxyribose moiety of DNA is replaced with a six-membered morpholine ring and the charged phosphodiester internucleoside linkages are replaced with neutral phosphorodiamidate linkages. PMO internalization in uptake-permissive cells has been observed to be specific, saturable, and energy-dependent, suggesting a receptor-mediated uptake mechanism. Understanding PMO transport should facilitate the design of more effective synthetic antisense oligomers as therapeutic agents.

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Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2009

Volume

542

Start / End Page

351 / 361

Location

United States

Related Subject Headings

  • Neoplasms
  • Neoplasm Proteins
  • Morpholinos
  • Morpholines
  • Humans
  • Gene Transfer Techniques
  • Developmental Biology
  • Cell Line, Tumor
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

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Devi, G. R. (2009). Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells. Methods Mol Biol, 542, 351–361. https://doi.org/10.1007/978-1-59745-561-9_19
Devi, Gayathri R. “Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells.Methods Mol Biol 542 (2009): 351–61. https://doi.org/10.1007/978-1-59745-561-9_19.
Devi, Gayathri R. “Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells.Methods Mol Biol, vol. 542, 2009, pp. 351–61. Pubmed, doi:10.1007/978-1-59745-561-9_19.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2009

Volume

542

Start / End Page

351 / 361

Location

United States

Related Subject Headings

  • Neoplasms
  • Neoplasm Proteins
  • Morpholinos
  • Morpholines
  • Humans
  • Gene Transfer Techniques
  • Developmental Biology
  • Cell Line, Tumor
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology