Scholars@Duke publication: Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.

Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.

Publication , Journal Article

Milano, CA; Dolber, PC; Rockman, HA; Bond, RA; Venable, ME; Allen, LF; Lefkowitz, RJ

Published in: Proc Natl Acad Sci U S A

October 11, 1994

Published version (DOI) Open Access Copy (Duke) Link to item

Transgenic mice were generated by using the alpha-myosin heavy chain promoter coupled to the coding sequence of a constitutively active mutant alpha 1B-adrenergic receptor (AR). These transgenic animals demonstrated cardiac-specific expression of this alpha 1-AR with resultant activation of phospholipase C as shown by increased myocardial diacylglycerol content. A phenotype consistent with cardiac hypertrophy developed in adult transgenic mice with increased heart/body weight ratios, myocyte cross-sectional areas, and ventricular atrial natriuretic factor mRNA levels relative to nontransgenic controls. These transgenic animals may provide insight into the biochemical triggers that induce hypertrophy in cardiac disease and serve as a convenient experimental model for studies of this condition.

Duke Scholars

Author Robert J. Lefkowitz Medicine, Cardiology

Author Carmelo Alessio Milano Surgery, Cardiovascular and Thoracic Surgery

Author Howard Allan Rockman Medicine, Cardiology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.21.10109

ISSN

0027-8424

Publication Date

October 11, 1994

Volume

Issue

Start / End Page

10109 / 10113

Location

United States

Related Subject Headings

Type C Phospholipases
Reference Values
Receptors, Adrenergic, alpha-1
Radioligand Assay
RNA, Messenger
Promoter Regions, Genetic
Point Mutation
Organ Size
Myosins
Myocardium

Citation

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Chicago

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NLM

Milano, C. A., Dolber, P. C., Rockman, H. A., Bond, R. A., Venable, M. E., Allen, L. F., & Lefkowitz, R. J. (1994). Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. Proc Natl Acad Sci U S A, 91(21), 10109–10113. https://doi.org/10.1073/pnas.91.21.10109

Milano, C. A., P. C. Dolber, H. A. Rockman, R. A. Bond, M. E. Venable, L. F. Allen, and R. J. Lefkowitz. “Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.” Proc Natl Acad Sci U S A 91, no. 21 (October 11, 1994): 10109–13. https://doi.org/10.1073/pnas.91.21.10109.

Milano CA, Dolber PC, Rockman HA, Bond RA, Venable ME, Allen LF, et al. Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10109–13.

Milano, C. A., et al. “Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.” Proc Natl Acad Sci U S A, vol. 91, no. 21, Oct. 1994, pp. 10109–13. Pubmed, doi:10.1073/pnas.91.21.10109.

Milano CA, Dolber PC, Rockman HA, Bond RA, Venable ME, Allen LF, Lefkowitz RJ. Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10109–10113.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.21.10109

ISSN

0027-8424

Publication Date

October 11, 1994

Volume

Issue

Start / End Page

10109 / 10113

Location

United States

Related Subject Headings

Type C Phospholipases
Reference Values
Receptors, Adrenergic, alpha-1
Radioligand Assay
RNA, Messenger
Promoter Regions, Genetic
Point Mutation
Organ Size
Myosins
Myocardium