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Dicistronic polioviruses as expression vectors for foreign genes.

Publication ,  Journal Article
Alexander, L; Lu, HH; Gromeier, M; Wimmer, E
Published in: AIDS Res Hum Retroviruses
1994

We have made use of certain novel genetic elements of picornaviruses termed internal ribosomal entry sites (IRES) to construct a viral RNA with the following genetic order: PV 5' NTR-EMCV IRES-PV ORF-3' NTR (PV, poliovirus; NTR, nontranslated region; EMCV, encephalomyocarditis virus; ORF, open reading frame). Transfection of this RNA into HeLa cells yielded a poliovirus (W1-PNENPO) that contained two heterologous IRES elements (type 1 IRES of PV; type 2 IRES of EMCV) in tandem. The insertion of foreign coding sequences into the genome of W1-PNENPO between the IRES elements yielded viable polioviruses with the gene order PV 5' NTR-foreign ORF-EMCV IRES-PV ORF-3' NTR. The foreign ORFs we have employed in this study included the coding region for chloramphenicol acetyltransferase (CAT), or segments of either luciferase or the HIV-1 envelope glycoprotein gp120. W1-PV/V3-3, a dicistronic poliovirus that contained HIV-1-specific sequences that included the V3 domain of gp120, was used to infect transgenic mice (PVR+) that were engineered to express the poliovirus receptor. The genetic stability of the dicistronic viruses and the HIV-1-specific immune response in PVR+ mice after infection with these novel agents are discussed.

Duke Scholars

Published In

AIDS Res Hum Retroviruses

ISSN

0889-2229

Publication Date

1994

Volume

10 Suppl 2

Start / End Page

S57 / S60

Location

United States

Related Subject Headings

  • Virology
  • Transfection
  • Poliovirus
  • Peptide Fragments
  • Open Reading Frames
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Humans
  • Hela Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Alexander, L., Lu, H. H., Gromeier, M., & Wimmer, E. (1994). Dicistronic polioviruses as expression vectors for foreign genes. AIDS Res Hum Retroviruses, 10 Suppl 2, S57–S60.
Alexander, L., H. H. Lu, M. Gromeier, and E. Wimmer. “Dicistronic polioviruses as expression vectors for foreign genes.AIDS Res Hum Retroviruses 10 Suppl 2 (1994): S57–60.
Alexander L, Lu HH, Gromeier M, Wimmer E. Dicistronic polioviruses as expression vectors for foreign genes. AIDS Res Hum Retroviruses. 1994;10 Suppl 2:S57–60.
Alexander, L., et al. “Dicistronic polioviruses as expression vectors for foreign genes.AIDS Res Hum Retroviruses, vol. 10 Suppl 2, 1994, pp. S57–60.
Alexander L, Lu HH, Gromeier M, Wimmer E. Dicistronic polioviruses as expression vectors for foreign genes. AIDS Res Hum Retroviruses. 1994;10 Suppl 2:S57–S60.
Journal cover image

Published In

AIDS Res Hum Retroviruses

ISSN

0889-2229

Publication Date

1994

Volume

10 Suppl 2

Start / End Page

S57 / S60

Location

United States

Related Subject Headings

  • Virology
  • Transfection
  • Poliovirus
  • Peptide Fragments
  • Open Reading Frames
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Humans
  • Hela Cells