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Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants.

Publication ,  Journal Article
Gromeier, M; Alexander, L; Wimmer, E
Published in: Proc Natl Acad Sci U S A
March 19, 1996

Neuropathogenicity of poliovirus can be attenuated by mutations in the internal ribosomal entry site (IRES) within the 5' nontranslated region of its genome. The Sabin vaccine strains used in prevention of poliomyelitis carry such mutations in their IRES elements. In addition, mutations within the structural and nonstructural proteins of Sabin strains may equally contribute to the attenuation phenotype. Despite their effectiveness as vaccines, the Sabin strains retain a neuropathogenic potential in animal models for poliomyelitis and, at a very low rate, they can cause poliomyelitis in vaccine recipients. The elimination of the neurocytopathic phenotype was achieved through the exchange of the entire poliovirus IRES with its counterpart from human rhinovirus type 2 without affecting growth properties in nonneuronal cells. The attenuating effect of the human rhinovirus type 2 IRES within the context of a poliovirus genome has been mapped to the 3' portion of this genetic element.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 19, 1996

Volume

93

Issue

6

Start / End Page

2370 / 2375

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Viral
  • RNA, Messenger
  • Poliovirus
  • Peptide Chain Initiation, Translational
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Mice
  • Humans
 

Citation

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Gromeier, M., Alexander, L., & Wimmer, E. (1996). Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc Natl Acad Sci U S A, 93(6), 2370–2375. https://doi.org/10.1073/pnas.93.6.2370
Gromeier, M., L. Alexander, and E. Wimmer. “Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants.Proc Natl Acad Sci U S A 93, no. 6 (March 19, 1996): 2370–75. https://doi.org/10.1073/pnas.93.6.2370.
Gromeier M, Alexander L, Wimmer E. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2370–5.
Gromeier, M., et al. “Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants.Proc Natl Acad Sci U S A, vol. 93, no. 6, Mar. 1996, pp. 2370–75. Pubmed, doi:10.1073/pnas.93.6.2370.
Gromeier M, Alexander L, Wimmer E. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2370–2375.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 19, 1996

Volume

93

Issue

6

Start / End Page

2370 / 2375

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Viral
  • RNA, Messenger
  • Poliovirus
  • Peptide Chain Initiation, Translational
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Mice
  • Humans