Scholars@Duke publication: Genetic recombination of poliovirus in a cell-free system.

Genetic recombination of poliovirus in a cell-free system.

Publication , Journal Article

Duggal, R; Cuconati, A; Gromeier, M; Wimmer, E

Published in: Proc Natl Acad Sci U S A

December 9, 1997

Genetic recombination of plus-strand RNA viruses is an important process for promoting genetic variation. By using genetically marked poliovirus RNAs, we have demonstrated that genetic recombination can occur in a cell-free system that generates infective virus from added poliovirus RNA. Recombinant polioviruses were isolated, and the region of crossing over was roughly mapped. Recombinants could be isolated even under conditions where the yield of viruses from one of the parental RNAs was depressed to levels comparable to or less than the yield of recombinant viruses, an observation suggesting that only one of the recombining RNAs needs to be replication-competent. The generation of poliovirus recombinants in a cell-free system offers new possibilities for studying recombination and evolution of RNA viruses.

Duke Scholars

Author Matthias Gromeier Neurosurgery, Neuro-Oncology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.25.13786

ISSN

0027-8424

Publication Date

December 9, 1997

Volume

Issue

Start / End Page

13786 / 13791

Location

United States

Related Subject Headings

Virus Replication
Recombination, Genetic
RNA, Viral
Polymerase Chain Reaction
Poliovirus
Phenotype
In Vitro Techniques
Humans
Genetic Variation
Genetic Markers

Citation

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Chicago

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MLA

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Duggal, R., Cuconati, A., Gromeier, M., & Wimmer, E. (1997). Genetic recombination of poliovirus in a cell-free system. Proc Natl Acad Sci U S A, 94(25), 13786–13791. https://doi.org/10.1073/pnas.94.25.13786

Duggal, R., A. Cuconati, M. Gromeier, and E. Wimmer. “Genetic recombination of poliovirus in a cell-free system.” Proc Natl Acad Sci U S A 94, no. 25 (December 9, 1997): 13786–91. https://doi.org/10.1073/pnas.94.25.13786.

Duggal R, Cuconati A, Gromeier M, Wimmer E. Genetic recombination of poliovirus in a cell-free system. Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13786–91.

Duggal, R., et al. “Genetic recombination of poliovirus in a cell-free system.” Proc Natl Acad Sci U S A, vol. 94, no. 25, Dec. 1997, pp. 13786–91. Pubmed, doi:10.1073/pnas.94.25.13786.

Duggal R, Cuconati A, Gromeier M, Wimmer E. Genetic recombination of poliovirus in a cell-free system. Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13786–13791.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.25.13786

ISSN

0027-8424

Publication Date

December 9, 1997

Volume

Issue

Start / End Page

13786 / 13791

Location

United States

Related Subject Headings

Virus Replication
Recombination, Genetic
RNA, Viral
Polymerase Chain Reaction
Poliovirus
Phenotype
In Vitro Techniques
Humans
Genetic Variation
Genetic Markers