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TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.

Publication ,  Journal Article
Song, J; Bishop, BL; Li, G; Grady, R; Stapleton, A; Abraham, SN
Published in: Proc Natl Acad Sci U S A
September 1, 2009

Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR)4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

September 1, 2009

Volume

106

Issue

35

Start / End Page

14966 / 14971

Location

United States

Related Subject Headings

  • rab GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Urinary Bladder
  • Toll-Like Receptor 4
  • Lipopolysaccharides
  • Humans
  • Exocytosis
  • Escherichia coli
  • Epithelial Cells
  • Cyclic AMP
 

Citation

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Song, J., Bishop, B. L., Li, G., Grady, R., Stapleton, A., & Abraham, S. N. (2009). TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A, 106(35), 14966–14971. https://doi.org/10.1073/pnas.0900527106
Song, Jeongmin, Brian L. Bishop, Guojie Li, Richard Grady, Ann Stapleton, and Soman N. Abraham. “TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.Proc Natl Acad Sci U S A 106, no. 35 (September 1, 2009): 14966–71. https://doi.org/10.1073/pnas.0900527106.
Song J, Bishop BL, Li G, Grady R, Stapleton A, Abraham SN. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14966–71.
Song, Jeongmin, et al. “TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.Proc Natl Acad Sci U S A, vol. 106, no. 35, Sept. 2009, pp. 14966–71. Pubmed, doi:10.1073/pnas.0900527106.
Song J, Bishop BL, Li G, Grady R, Stapleton A, Abraham SN. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14966–14971.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

September 1, 2009

Volume

106

Issue

35

Start / End Page

14966 / 14971

Location

United States

Related Subject Headings

  • rab GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Urinary Bladder
  • Toll-Like Receptor 4
  • Lipopolysaccharides
  • Humans
  • Exocytosis
  • Escherichia coli
  • Epithelial Cells
  • Cyclic AMP