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Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins.

Publication ,  Journal Article
Lee, Y; Hami, D; De Val, S; Kagermeier-Schenk, B; Wills, AA; Black, BL; Weidinger, G; Poss, KD
Published in: Dev Biol
July 15, 2009

Appendage regeneration in salamanders and fish occurs through formation and maintenance of a mass of progenitor tissue called the blastema. A dedicated epidermis overlays the blastema and is required for its proliferation and patterning, yet this interaction is poorly understood. Here, we identified molecularly and functionally distinct compartments within the basal epidermal layer during zebrafish fin regeneration. Proximal epidermal subtypes express the transcription factor lef1 and the blastemal mitogen shh, while distal subtypes express the Fgf target gene pea3 and wnt5b, an inhibitor of blastemal proliferation. Ectopic overexpression of wnt5b reduced shh expression, while pharmacologic introduction of a Hh pathway agonist partially rescued blastemal proliferation during wnt5b overexpression. Loss- and gain-of-function approaches indicate that Fgf signaling promotes shh expression in proximal epidermis, while Fgf/Ras signaling restricts shh expression from distal epidermis through induction of pea3 expression and maintenance of wnt5b. Thus, the fin wound epidermis spatially confines Hh signaling through the activity of Fgf and Wnt pathways, impacting blastemal proliferation during regenerative outgrowth.

Duke Scholars

Published In

Dev Biol

DOI

EISSN

1095-564X

Publication Date

July 15, 2009

Volume

331

Issue

2

Start / End Page

270 / 280

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Transcription Factors
  • Signal Transduction
  • Regeneration
  • Fibroblast Growth Factors
  • Extremities
  • Epidermis
  • Developmental Biology
  • Body Patterning
 

Citation

APA
Chicago
ICMJE
MLA
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Lee, Y., Hami, D., De Val, S., Kagermeier-Schenk, B., Wills, A. A., Black, B. L., … Poss, K. D. (2009). Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins. Dev Biol, 331(2), 270–280. https://doi.org/10.1016/j.ydbio.2009.05.545
Lee, Yoonsung, Danyal Hami, Sarah De Val, Birgit Kagermeier-Schenk, Airon A. Wills, Brian L. Black, Gilbert Weidinger, and Kenneth D. Poss. “Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins.Dev Biol 331, no. 2 (July 15, 2009): 270–80. https://doi.org/10.1016/j.ydbio.2009.05.545.
Lee Y, Hami D, De Val S, Kagermeier-Schenk B, Wills AA, Black BL, et al. Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins. Dev Biol. 2009 Jul 15;331(2):270–80.
Lee, Yoonsung, et al. “Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins.Dev Biol, vol. 331, no. 2, July 2009, pp. 270–80. Pubmed, doi:10.1016/j.ydbio.2009.05.545.
Lee Y, Hami D, De Val S, Kagermeier-Schenk B, Wills AA, Black BL, Weidinger G, Poss KD. Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins. Dev Biol. 2009 Jul 15;331(2):270–280.
Journal cover image

Published In

Dev Biol

DOI

EISSN

1095-564X

Publication Date

July 15, 2009

Volume

331

Issue

2

Start / End Page

270 / 280

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Transcription Factors
  • Signal Transduction
  • Regeneration
  • Fibroblast Growth Factors
  • Extremities
  • Epidermis
  • Developmental Biology
  • Body Patterning