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Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells.

Publication ,  Journal Article
Shinohara, ML; Kim, H-J; Kim, J-H; Garcia, VA; Cantor, H
Published in: Proc Natl Acad Sci U S A
May 20, 2008

Osteopontin (Opn) contributes to diverse biological processes that include immune responses, vascularization, and bone formation. Until recently, studies describing the activities of Opn have focused on the cytokine-like properties of the secreted protein. Here, we show that alternative translation of a single Opn mRNA species generates a secreted and intracellular isoform. Utilization of a 5' canonical translation start site generates a protein that includes an N-terminal signal sequence allowing targeting to secretory vesicles and cytokine secretion, whereas usage of a downstream start site generates a shortened protein that lacks the N-terminal signal sequence and localizes mainly to cytoplasm. The coordinated action of these Opn gene products regulates the functional phenotype of subsets of dendritic cells.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 20, 2008

Volume

105

Issue

20

Start / End Page

7235 / 7239

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Protein Structure, Tertiary
  • Protein Isoforms
  • Phenotype
  • Osteopontin
  • Models, Genetic
  • Models, Biological
  • Humans
  • Gene Expression Regulation
 

Citation

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Shinohara, M. L., Kim, H.-J., Kim, J.-H., Garcia, V. A., & Cantor, H. (2008). Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells. Proc Natl Acad Sci U S A, 105(20), 7235–7239. https://doi.org/10.1073/pnas.0802301105
Shinohara, Mari L., Hye-Jung Kim, June-Ho Kim, Virgilio A. Garcia, and Harvey Cantor. “Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells.Proc Natl Acad Sci U S A 105, no. 20 (May 20, 2008): 7235–39. https://doi.org/10.1073/pnas.0802301105.
Shinohara ML, Kim H-J, Kim J-H, Garcia VA, Cantor H. Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7235–9.
Shinohara, Mari L., et al. “Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells.Proc Natl Acad Sci U S A, vol. 105, no. 20, May 2008, pp. 7235–39. Pubmed, doi:10.1073/pnas.0802301105.
Shinohara ML, Kim H-J, Kim J-H, Garcia VA, Cantor H. Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7235–7239.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 20, 2008

Volume

105

Issue

20

Start / End Page

7235 / 7239

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Protein Structure, Tertiary
  • Protein Isoforms
  • Phenotype
  • Osteopontin
  • Models, Genetic
  • Models, Biological
  • Humans
  • Gene Expression Regulation