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Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis.

Publication ,  Journal Article
Halsell, SR; Chu, BI; Kiehart, DP
Published in: Genetics
July 2000

A dynamic actomyosin cytoskeleton drives many morphogenetic events. Conventional nonmuscle myosin-II (myosin) is a key chemomechanical motor that drives contraction of the actin cytoskeleton. We have explored the regulation of myosin activity by performing genetic screens to identify gene products that collaborate with myosin during Drosophila morphogenesis. Specifically, we screened for second-site noncomplementors of a mutation in the zipper gene that encodes the nonmuscle myosin-II heavy chain. We determined that a single missense mutation in the zipper(Ebr) allele gives rise to its sensitivity to second-site noncomplementation. We then identify the Rho signal transduction pathway as necessary for proper myosin function. First we show that a lethal P-element insertion interacts genetically with zipper. Subsequently we show that this second-site noncomplementing mutation disrupts the RhoGEF2 locus. Next, we show that two EMS-induced mutations, previously shown to interact genetically with zipper(Ebr), disrupt the RhoA locus. Further, we have identified their molecular lesions and determined that disruption of the carboxyl-terminal CaaX box gives rise to their mutant phenotype. Finally, we show that RhoA mutations themselves can be utilized in genetic screens. Biochemical and cell culture analyses suggest that Rho signal transduction regulates the activity of myosin. Our studies provide direct genetic proof of the biological relevance of regulation of myosin by Rho signal transduction in an intact metazoan.

Duke Scholars

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

July 2000

Volume

155

Issue

3

Start / End Page

1253 / 1265

Related Subject Headings

  • rhoA GTP-Binding Protein
  • Signal Transduction
  • Rho Guanine Nucleotide Exchange Factors
  • Protein Structure, Tertiary
  • Physical Chromosome Mapping
  • Myosins
  • Myosin Heavy Chains
  • Mutation, Missense
  • Morphogenesis
  • Membrane Proteins
 

Citation

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Halsell, S. R., Chu, B. I., & Kiehart, D. P. (2000). Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis. Genetics, 155(3), 1253–1265. https://doi.org/10.1093/genetics/155.3.1253
Halsell, S. R., B. I. Chu, and D. P. Kiehart. “Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis.Genetics 155, no. 3 (July 2000): 1253–65. https://doi.org/10.1093/genetics/155.3.1253.
Halsell, S. R., et al. “Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis.Genetics, vol. 155, no. 3, July 2000, pp. 1253–65. Epmc, doi:10.1093/genetics/155.3.1253.

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

July 2000

Volume

155

Issue

3

Start / End Page

1253 / 1265

Related Subject Headings

  • rhoA GTP-Binding Protein
  • Signal Transduction
  • Rho Guanine Nucleotide Exchange Factors
  • Protein Structure, Tertiary
  • Physical Chromosome Mapping
  • Myosins
  • Myosin Heavy Chains
  • Mutation, Missense
  • Morphogenesis
  • Membrane Proteins