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Idiotypic analysis of a monoclonal anti-Sm antibody.

Publication ,  Journal Article
Pisetsky, DS; Lerner, EA
Published in: J Immunol
October 1982

Among murine models of autoimmunity, MRL mice are unique in their expression of antibodies to the nuclear antigen Sm. To assess genetic mechanisms in the control of this response, the idiotypes borne by a monoclonal anti-Sm antibody of MRL-Ipr/Ipr origin were investigated. Rabbit antisera were prepared against Y2, a hybridoma product with anti-Sm activity, and were rendered specific for idiotype by extensive absorption with normal globulins from BALB/c mice. In assays of idiotype by an inhibition ELISA, Y2 was shown to share idiotypes with Y12, another monoclonal anti-Sm derived from the same fusion as Y2; other monoclonal autoantibodies of MRL origin but different antigenic specificity failed to display idiotype activity in this assay. The presence of other anti-idiotypic specificities was revealed by absorption and elution of the anti-idiotype from an MRL globulin column; sera from both anti-Sm-positive and negative mice demonstrated these idiotypes. These results suggest that the predominant specificities detected by the anti-idiotype were unique to the monoclonal antibodies of the same animal, although there was also activity to idiotypes not related to anti-Sm binding molecules.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1982

Volume

129

Issue

4

Start / End Page

1489 / 1492

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes
  • Epitopes
  • Autoantigens
  • Autoantibodies
  • Antigens
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pisetsky, D. S., & Lerner, E. A. (1982). Idiotypic analysis of a monoclonal anti-Sm antibody. J Immunol, 129(4), 1489–1492.
Pisetsky, D. S., and E. A. Lerner. “Idiotypic analysis of a monoclonal anti-Sm antibody.J Immunol 129, no. 4 (October 1982): 1489–92.
Pisetsky DS, Lerner EA. Idiotypic analysis of a monoclonal anti-Sm antibody. J Immunol. 1982 Oct;129(4):1489–92.
Pisetsky, D. S., and E. A. Lerner. “Idiotypic analysis of a monoclonal anti-Sm antibody.J Immunol, vol. 129, no. 4, Oct. 1982, pp. 1489–92.
Pisetsky DS, Lerner EA. Idiotypic analysis of a monoclonal anti-Sm antibody. J Immunol. 1982 Oct;129(4):1489–1492.

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1982

Volume

129

Issue

4

Start / End Page

1489 / 1492

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes
  • Epitopes
  • Autoantigens
  • Autoantibodies
  • Antigens
  • Antibodies, Monoclonal