Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Effect of cytofectins on the immune response of murine macrophages to mammalian DNA.

Publication ,  Journal Article
Zhu, F-G; Reich, CF; Pisetsky, DS
Published in: Immunology
June 2003

DNA, depending on base sequence, can induce a wide range of immune responses. While bacterial DNA is stimulatory, mammalian DNA is inactive alone and can, moreover, inhibit the response to bacterial DNA. To determine whether the mode of cell entry affects the immune properties of mammalian DNA, we have investigated the effects of the cytofectin agents Fugene 6 (Roche Diagnostics Corp., Indianapolis, IN), Lipofectin and Lipofectamine (Life Technologies, Grand Island, NY) on the responses of murine macrophages to DNA from calf thymus and human placenta. Whereas calf thymus and human placenta DNA alone failed to stimulate J774 or RAW264.7 cell lines or bone marrow-derived macrophages, these DNAs in complexes with cytofectin agents stimulated macrophages to produce nitric oxide but not interleukin 12. Both single-stranded and double-stranded DNAs were active in the presence of cytofectins. Macrophage activation by the DNA-cytofectin complexes was reduced by chloroquine, suggesting a role of endosomal acidification in activation. As shown by flow cytometry and confocal microscopy, the cytofectins caused an increase in the uptake of DNA into cells. Our findings indicate that macrophages vary in their response to DNA depending on uptake pathway, suggesting that activation by DNA reflects not only sequence but also context or intracellular location.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Immunology

DOI

ISSN

0019-2805

Publication Date

June 2003

Volume

109

Issue

2

Start / End Page

255 / 262

Location

England

Related Subject Headings

  • Polydeoxyribonucleotides
  • Phosphatidylethanolamines
  • Nitric Oxide
  • Microscopy, Confocal
  • Mice, Inbred BALB C
  • Mice
  • Macrophages
  • Macrophage Activation
  • Liposomes
  • Lipids
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhu, F.-G., Reich, C. F., & Pisetsky, D. S. (2003). Effect of cytofectins on the immune response of murine macrophages to mammalian DNA. Immunology, 109(2), 255–262. https://doi.org/10.1046/j.1365-2567.2003.01653.x
Zhu, Fu-Gang, Charles F. Reich, and David S. Pisetsky. “Effect of cytofectins on the immune response of murine macrophages to mammalian DNA.Immunology 109, no. 2 (June 2003): 255–62. https://doi.org/10.1046/j.1365-2567.2003.01653.x.
Zhu F-G, Reich CF, Pisetsky DS. Effect of cytofectins on the immune response of murine macrophages to mammalian DNA. Immunology. 2003 Jun;109(2):255–62.
Zhu, Fu-Gang, et al. “Effect of cytofectins on the immune response of murine macrophages to mammalian DNA.Immunology, vol. 109, no. 2, June 2003, pp. 255–62. Pubmed, doi:10.1046/j.1365-2567.2003.01653.x.
Zhu F-G, Reich CF, Pisetsky DS. Effect of cytofectins on the immune response of murine macrophages to mammalian DNA. Immunology. 2003 Jun;109(2):255–262.

Published In

Immunology

DOI

ISSN

0019-2805

Publication Date

June 2003

Volume

109

Issue

2

Start / End Page

255 / 262

Location

England

Related Subject Headings

  • Polydeoxyribonucleotides
  • Phosphatidylethanolamines
  • Nitric Oxide
  • Microscopy, Confocal
  • Mice, Inbred BALB C
  • Mice
  • Macrophages
  • Macrophage Activation
  • Liposomes
  • Lipids