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Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA.

Publication ,  Journal Article
Gilkeson, GS; Grudier, JP; Karounos, DG; Pisetsky, DS
Published in: J Immunol
March 1, 1989

Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 1, 1989

Volume

142

Issue

5

Start / End Page

1482 / 1486

Location

United States

Related Subject Headings

  • Micrococcus
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Kinetics
  • Immunology
  • Escherichia coli
  • DNA, Bacterial
  • DNA
 

Citation

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ICMJE
MLA
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Gilkeson, G. S., Grudier, J. P., Karounos, D. G., & Pisetsky, D. S. (1989). Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA. J Immunol, 142(5), 1482–1486.
Gilkeson, G. S., J. P. Grudier, D. G. Karounos, and D. S. Pisetsky. “Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA.J Immunol 142, no. 5 (March 1, 1989): 1482–86.
Gilkeson GS, Grudier JP, Karounos DG, Pisetsky DS. Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA. J Immunol. 1989 Mar 1;142(5):1482–6.
Gilkeson, G. S., et al. “Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA.J Immunol, vol. 142, no. 5, Mar. 1989, pp. 1482–86.
Gilkeson GS, Grudier JP, Karounos DG, Pisetsky DS. Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA. J Immunol. 1989 Mar 1;142(5):1482–1486.

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 1, 1989

Volume

142

Issue

5

Start / End Page

1482 / 1486

Location

United States

Related Subject Headings

  • Micrococcus
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Kinetics
  • Immunology
  • Escherichia coli
  • DNA, Bacterial
  • DNA