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Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency.

Publication ,  Journal Article
Cohen, PL; Shores, EW; Rapoport, R; Caster, S; Eisenberg, RA; Pisetsky, DS
Published in: Cell Immunol
December 1985

Individual MRL-lpr mice vary in their capacity to generate anti-Sm autoantibodies spontaneously. We have compared the frequency of B-cell precursors for this autoantibody in serologically negative and serologically positive MRL-lpr mice, and in normals. Anti-Sm precursors were present in a frequency of approximately 1 per 10-30,000 in spleen cell cultures from anti-Sm positive mice, but were undetectable when spleen cells from serologically negative MRL-lpr mice or from normal mice were examined. Despite LPS stimulation, neither IgM nor IgG precursors could be detected. In parallel cultures, in contrast, anti-DNA autoantibody precursors were readily detected. The results thus indicate that, for the lupus-specific autoantibodies, the absence of antibody in autoimmune mice reflects a deficit in precursor B lymphocytes rather than an active regulatory mechanism. It is suggested that the generation of anti-Sm may reflect a low-probability random event in the generation of B-cell diversity.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

December 1985

Volume

96

Issue

2

Start / End Page

448 / 454

Location

Netherlands

Related Subject Headings

  • snRNP Core Proteins
  • Spleen
  • Ribonucleoproteins, Small Nuclear
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cohen, P. L., Shores, E. W., Rapoport, R., Caster, S., Eisenberg, R. A., & Pisetsky, D. S. (1985). Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency. Cell Immunol, 96(2), 448–454. https://doi.org/10.1016/0008-8749(85)90376-4
Cohen, P. L., E. W. Shores, R. Rapoport, S. Caster, R. A. Eisenberg, and D. S. Pisetsky. “Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency.Cell Immunol 96, no. 2 (December 1985): 448–54. https://doi.org/10.1016/0008-8749(85)90376-4.
Cohen PL, Shores EW, Rapoport R, Caster S, Eisenberg RA, Pisetsky DS. Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency. Cell Immunol. 1985 Dec;96(2):448–54.
Cohen, P. L., et al. “Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency.Cell Immunol, vol. 96, no. 2, Dec. 1985, pp. 448–54. Pubmed, doi:10.1016/0008-8749(85)90376-4.
Cohen PL, Shores EW, Rapoport R, Caster S, Eisenberg RA, Pisetsky DS. Anti-Sm autoantibodies in MRL mice: analysis of precursor frequency. Cell Immunol. 1985 Dec;96(2):448–454.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

December 1985

Volume

96

Issue

2

Start / End Page

448 / 454

Location

Netherlands

Related Subject Headings

  • snRNP Core Proteins
  • Spleen
  • Ribonucleoproteins, Small Nuclear
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G