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Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice.

Publication ,  Journal Article
Warren, RW; Sailstad, DM; Caster, SA; Pisetsky, DS
Published in: Arthritis Rheum
May 1984

The binding properties of B6-lpr/lpr anti-DNA monoclonal antibodies were characterized to evaluate the influence of genetic background on the diversity and specificity of lpr-induced autoantibody responses. Six anti-DNA antibodies were produced from fusions with B6-lpr/lpr mice, while another was obtained from a fusion with a B6-+/+ mouse immunized with lipopolysaccharide. Each antibody bound single-stranded DNA in preference to double-stranded DNA, with variation of over 300-fold in relative binding activities. In terms of binding to a panel of synthetic polynucleotides, each antibody exhibited a unique antigenic specificity. This binding, however, was not dependent on recognition of a unique base or sugar moiety, since individual antibodies bound polymers of dissimilar composition. These results suggest a diversity of binding reactions for B6-lpr/lpr anti-DNA antibodies, with a clonal repertoire similar to that of mice from autoimmune backgrounds.

Duke Scholars

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

May 1984

Volume

27

Issue

5

Start / End Page

545 / 551

Location

United States

Related Subject Headings

  • Polynucleotides
  • Oligonucleotides
  • Mice, Inbred Strains
  • Mice
  • Male
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Binding Sites, Antibody
  • Autoantibodies
 

Citation

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ICMJE
MLA
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Warren, R. W., Sailstad, D. M., Caster, S. A., & Pisetsky, D. S. (1984). Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice. Arthritis Rheum, 27(5), 545–551. https://doi.org/10.1002/art.1780270510
Warren, R. W., D. M. Sailstad, S. A. Caster, and D. S. Pisetsky. “Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice.Arthritis Rheum 27, no. 5 (May 1984): 545–51. https://doi.org/10.1002/art.1780270510.
Warren RW, Sailstad DM, Caster SA, Pisetsky DS. Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice. Arthritis Rheum. 1984 May;27(5):545–51.
Warren, R. W., et al. “Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice.Arthritis Rheum, vol. 27, no. 5, May 1984, pp. 545–51. Pubmed, doi:10.1002/art.1780270510.
Warren RW, Sailstad DM, Caster SA, Pisetsky DS. Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice. Arthritis Rheum. 1984 May;27(5):545–551.
Journal cover image

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

May 1984

Volume

27

Issue

5

Start / End Page

545 / 551

Location

United States

Related Subject Headings

  • Polynucleotides
  • Oligonucleotides
  • Mice, Inbred Strains
  • Mice
  • Male
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Binding Sites, Antibody
  • Autoantibodies