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Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity.

Publication ,  Journal Article
Pisetsky, DS; Caster, SA
Published in: Clin Immunol Immunopathol
March 1984

The idiotypic determinants borne by Y2, a monoclonal anti-SM antibody of MRL mouse strain origin, were investigated to elucidate mechanisms for the generation of autoantibodies. Using an ELISA assay, a rabbit anti-Y2 anti-idiotypic antiserum was tested for binding to other MRL autoantibodies to identify cross-reactive determinants. In this way, a shared idiotype was demonstrated for Y2 and 4K1, 4k1 is an IgG2bK monoclonal antibody derived from a fusion with spleen cells of an MRL-lpr/lpr mouse expressing anti-Sm. 4K1 displayed an antigenic specificity distinct from anti-Sm, however, with reactivity by fluorescent antinuclear antibody analysis to a subcellular component localized to the perinuclear region. The expression of the Y2-4K1 idiotype was assessed using a sensitive inhibition ELISA. The Y2-4K1 determinant was present in sera of MRL-lpr/lpr mice irrespective of the presence of anti-Sm. Sera of some but not all normal mouse strains also demonstrated this determinant. These results indicate that while some idiotypes may be associated with autoantibodies, they are not restricted to the autoimmune setting. This pattern of expression suggests that autoantibodies are part of larger idiotype-bearing antibody families with structural similarity to antibodies produced by normal mice. The autoimmune setting may promote the emergence of idiotype-bearing molecules with autoantibody activity and aberrant regulation.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

March 1984

Volume

30

Issue

3

Start / End Page

461 / 468

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Mice, Inbred Strains
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice, Inbred BALB C
  • Mice, Inbred AKR
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes
 

Citation

APA
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ICMJE
MLA
NLM
Pisetsky, D. S., & Caster, S. A. (1984). Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity. Clin Immunol Immunopathol, 30(3), 461–468. https://doi.org/10.1016/0090-1229(84)90031-x
Pisetsky, D. S., and S. A. Caster. “Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity.Clin Immunol Immunopathol 30, no. 3 (March 1984): 461–68. https://doi.org/10.1016/0090-1229(84)90031-x.
Pisetsky DS, Caster SA. Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity. Clin Immunol Immunopathol. 1984 Mar;30(3):461–8.
Pisetsky, D. S., and S. A. Caster. “Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity.Clin Immunol Immunopathol, vol. 30, no. 3, Mar. 1984, pp. 461–68. Pubmed, doi:10.1016/0090-1229(84)90031-x.
Pisetsky DS, Caster SA. Idiotypic cross-reaction between MRL monoclonal autoantibodies with different antigen specificity. Clin Immunol Immunopathol. 1984 Mar;30(3):461–468.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

March 1984

Volume

30

Issue

3

Start / End Page

461 / 468

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Mice, Inbred Strains
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice, Inbred BALB C
  • Mice, Inbred AKR
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes