Skip to main content

The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase.

Publication ,  Journal Article
Pisetsky, DS; Reich, CF
Published in: Clin Immunol Immunopathol
September 1994

To elucidate the interaction of anti-DNA antibodies with DNA, the reactivity of lupus sera with single-stranded fragments from calf thymus, Escherichia coli, and salmon testes DNA was investigated. These fragments were generated by digestion with the restriction enzyme HinfI and ranged in size from approximately 100-4000 bases. By ELISA using polystyrene microtiter plates, fragments from all three species were weakly antigenic compared to intact DNA. These fragments, however, were all antigenic when tested as inhibitors in competition-binding assays. The weak antigenicity of fragments could not be explained by poor adherence to the plates since fragments and intact DNA showed similar levels of binding as assessed using biotinylated preparations. Together, these results demonstrate that the antigenicity of DNA fragments is dramatically altered by solid-phase binding and suggest that constraints on topological or conformational rearrangements of DNA in the solid phase limit antibody interaction.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

September 1994

Volume

72

Issue

3

Start / End Page

350 / 356

Location

United States

Related Subject Headings

  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Antibody Affinity
  • Antibodies, Antinuclear
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pisetsky, D. S., & Reich, C. F. (1994). The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase. Clin Immunol Immunopathol, 72(3), 350–356. https://doi.org/10.1006/clin.1994.1152
Pisetsky, D. S., and C. F. Reich. “The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase.Clin Immunol Immunopathol 72, no. 3 (September 1994): 350–56. https://doi.org/10.1006/clin.1994.1152.
Pisetsky DS, Reich CF. The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase. Clin Immunol Immunopathol. 1994 Sep;72(3):350–6.
Pisetsky, D. S., and C. F. Reich. “The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase.Clin Immunol Immunopathol, vol. 72, no. 3, Sept. 1994, pp. 350–56. Pubmed, doi:10.1006/clin.1994.1152.
Pisetsky DS, Reich CF. The influence of DNA size on the binding of anti-DNA antibodies in the solid and fluid phase. Clin Immunol Immunopathol. 1994 Sep;72(3):350–356.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

September 1994

Volume

72

Issue

3

Start / End Page

350 / 356

Location

United States

Related Subject Headings

  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Antibody Affinity
  • Antibodies, Antinuclear
  • Animals