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Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice.

Publication ,  Journal Article
Warren, RW; Roths, JB; Murphy, ED; Pisetsky, DS
Published in: Cell Immunol
March 1984

The influence of the lpr gene on spontaneous and lipopolysaccharide (LPS)-induced immunoglobulin production was studied in B6 mice homozygous for the mutant lpr gene (B6-lpr/lpr). Male and female mice of this congenic strain were followed for 1 year and sera serially tested by the enzyme-linked immunosorbent assay (ELISA) for the production of antibodies to single-stranded DNA (anti-sDNA), immunoglobulin (anti-IgG), and keyhole limpet hemocyanin (anti-KLH), models of autoantibody and non-autoantibody responses, respectively. Female B6-lpr/lpr mice demonstrated marked spontaneous responses to all three antigens; the responses of male B6-lpr/lpr mice were significantly lower but still exceeded those of the congenic B6-+/+ controls. These results demonstrate a generalized influence of sex on lpr associated responses. To determine whether this sex difference could be demonstrated with other forms of B-cell activation, young B6-+/+ and B6-lpr/lpr male and female mice were immunized with lipopolysaccharide and the induced responses determined. This immunization caused significant increases in the IgM response only. The levels of the induced responses produced after LPS treatment were comparable for +/+ and lpr/lpr mice. These results indicate that the enhanced responsiveness of female mice to lpr action is not reflected in the polyclonal response to LPS, which, furthermore, was unaffected by the presence of lpr. The differential influence of sex on lpr and LPS-induced responses and their apparent independence suggests that lpr and LPS promote B-cell activation by dissimilar mechanisms.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

March 1984

Volume

84

Issue

1

Start / End Page

22 / 31

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Sex Characteristics
  • Poly T
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Lipopolysaccharides
  • Immunology
 

Citation

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Chicago
ICMJE
MLA
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Warren, R. W., Roths, J. B., Murphy, E. D., & Pisetsky, D. S. (1984). Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice. Cell Immunol, 84(1), 22–31. https://doi.org/10.1016/0008-8749(84)90073-x
Warren, R. W., J. B. Roths, E. D. Murphy, and D. S. Pisetsky. “Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice.Cell Immunol 84, no. 1 (March 1984): 22–31. https://doi.org/10.1016/0008-8749(84)90073-x.
Warren RW, Roths JB, Murphy ED, Pisetsky DS. Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice. Cell Immunol. 1984 Mar;84(1):22–31.
Warren, R. W., et al. “Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice.Cell Immunol, vol. 84, no. 1, Mar. 1984, pp. 22–31. Pubmed, doi:10.1016/0008-8749(84)90073-x.
Warren RW, Roths JB, Murphy ED, Pisetsky DS. Mechanisms of polyclonal B-cell activation in autoimmune B6-lpr/lpr mice. Cell Immunol. 1984 Mar;84(1):22–31.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

March 1984

Volume

84

Issue

1

Start / End Page

22 / 31

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Sex Characteristics
  • Poly T
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lymphocyte Activation
  • Lipopolysaccharides
  • Immunology