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IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice.

Publication ,  Journal Article
Pisetsky, DS; Darwin, BS; Reich, CF
Published in: Immunology
December 1990

To assess the specificity of anti-nuclear antibodies with cross-reactive rheumatoid factor (RF) activity, monoclonal anti-DNA and anti-Sm antibodies from MRL-lpr/lpr mice were tested for binding to a variety of IgG antigens. These antibodies had all been previously identified as binding heterologous IgG. By ELISA, antibodies in this panel all bound BALB/c myeloma proteins representing the different IgG subclasses, indicating broad reactivity with murine IgG as well as heterologous IgG. The determinant recognized by these antibodies was further investigated using the Fab, F(ab')2 and Fc fragments of both human as well as rabbit origin. All antibodies bound well to fragments as well as intact IgG antigens. These antibodies were further analysed by Western blotting, demonstrating that most bound to both heavy and light chains of human origin. Together, these observations suggest that some anti-nuclear antibodies bind a conserved antigenic determinant present widely on immunoglobulin chains. This determinant may represent a common sequence important in immunoglobulin domain structure.

Duke Scholars

Published In

Immunology

ISSN

0019-2805

Publication Date

December 1990

Volume

71

Issue

4

Start / End Page

586 / 591

Location

England

Related Subject Headings

  • Rheumatoid Factor
  • Mice, Inbred Strains
  • Mice
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin G
  • Immunoglobulin Fragments
  • Cross Reactions
  • Blotting, Western
  • Antibody Specificity
 

Citation

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Chicago
ICMJE
MLA
NLM
Pisetsky, D. S., Darwin, B. S., & Reich, C. F. (1990). IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice. Immunology, 71(4), 586–591.
Pisetsky, D. S., B. S. Darwin, and C. F. Reich. “IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice.Immunology 71, no. 4 (December 1990): 586–91.
Pisetsky DS, Darwin BS, Reich CF. IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice. Immunology. 1990 Dec;71(4):586–91.
Pisetsky, D. S., et al. “IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice.Immunology, vol. 71, no. 4, Dec. 1990, pp. 586–91.
Pisetsky DS, Darwin BS, Reich CF. IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice. Immunology. 1990 Dec;71(4):586–591.

Published In

Immunology

ISSN

0019-2805

Publication Date

December 1990

Volume

71

Issue

4

Start / End Page

586 / 591

Location

England

Related Subject Headings

  • Rheumatoid Factor
  • Mice, Inbred Strains
  • Mice
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin G
  • Immunoglobulin Fragments
  • Cross Reactions
  • Blotting, Western
  • Antibody Specificity