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Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus.

Publication ,  Journal Article
Ward, MM; Pisetsky, DS
Published in: Clin Immunol Immunopathol
November 1990

To determine whether age-related changes in immune function affect patterns of autoantibody production, we have examined the isotype and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus (SLE). Enzyme-linked immunosorbent assays (ELISA) were used to determine the frequencies of IgG and IgM antibodies to single-stranded DNA (ssDNA), Sm, and the 70K protein component of RNP in the sera of 53 patients with SLE older than age 60. The IgG subclass distributions and kappa/lambda ratios for each of these autoantibodies were also determined and compared to measurements performed on the sera of 53 young adult patients with SLE. The frequencies of autoantibodies of each specificity, except IgM anti-ss DNA antibodies, were higher among the young adult patients, although the magnitudes of the responses were similar in both age groups. IgG anti-Sm antibodies were composed of both IgG1 and IgG2 subclasses, while IgG anti-70K RNP and IgG anti-ssDNA were predominantly of the IgG1 subclass. There were no differences in the IgG subclass distributions of any of the three autoantibodies between the elderly and young adult patient sera. The kappa/lambda ratios for each of the three autoantibodies were similar to that present in total serum immunoglobulins, and kappa/lambda ratios of autoantibodies, standardized to the kappa/lambda ratios of serum, were not different between elderly and young adult groups. Few patient sera of either age group (9 elderly, 7 young adult) demonstrated even midly skewed light chain ratios in their autoantibody responses. Thus, despite developing in an immunological environment that may have altered the clonality and isotype distribution of their responses, the autoantibodies produced by elderly patients with SLE were qualitatively similar to autoantibodies of younger patients.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

November 1990

Volume

57

Issue

2

Start / End Page

280 / 296

Location

United States

Related Subject Headings

  • Middle Aged
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Autoantibodies
  • Aging
  • Aged
 

Citation

APA
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MLA
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Ward, M. M., & Pisetsky, D. S. (1990). Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus. Clin Immunol Immunopathol, 57(2), 280–296. https://doi.org/10.1016/0090-1229(90)90042-o
Ward, M. M., and D. S. Pisetsky. “Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus.Clin Immunol Immunopathol 57, no. 2 (November 1990): 280–96. https://doi.org/10.1016/0090-1229(90)90042-o.
Ward MM, Pisetsky DS. Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus. Clin Immunol Immunopathol. 1990 Nov;57(2):280–96.
Ward, M. M., and D. S. Pisetsky. “Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus.Clin Immunol Immunopathol, vol. 57, no. 2, Nov. 1990, pp. 280–96. Pubmed, doi:10.1016/0090-1229(90)90042-o.
Ward MM, Pisetsky DS. Heavy and light chain utilization in autoantibodies of elderly patients with systemic lupus erythematosus. Clin Immunol Immunopathol. 1990 Nov;57(2):280–296.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

November 1990

Volume

57

Issue

2

Start / End Page

280 / 296

Location

United States

Related Subject Headings

  • Middle Aged
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Autoantibodies
  • Aging
  • Aged