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The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains.

Publication ,  Journal Article
Warren, RW; Caster, SA; Roths, JB; Murphy, ED; Pisetsky, DS
Published in: Clin Immunol Immunopathol
April 1984

Spontaneous immunoglobulin production in four strains of lpr/lpr congenic mice was investigated to identify genetic interactions in lpr-induced polyclonal B cell activation. Sera were obtained from male and female lpr/lpr mice of the MRL, B6, C3H, and AKR strains as well as controls of +/+ genotypes. Antibody levels were compared at the time of peak response. Quantitative antibody determinations were performed by isotype-specific ELISA assays using responses to single-stranded DNA (sDNA), mouse IgG, rabbit IgG, and keyhole limpet hemocyanin as models. Among the strains studied there were significant differences in the antibody levels observed, with the strain producing highest levels dependent on the response measured. Thus, MRL-lpr/lpr produced the highest levels of IgG anti-sDNA while B6-lpr/lpr mice produced more anti-IgG than mice of other strains. Within each strain, the lpr gene appeared to affect the IgG more than the IgM response. A consistently high response by females was observed only in B6-lpr/lpr mice. These studies suggest that lpr-induced polyclonal B cell activation is influenced by the background genome with the extent of these genetic effects variable among responses.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1984

Volume

31

Issue

1

Start / End Page

65 / 77

Location

United States

Related Subject Headings

  • Rheumatoid Factor
  • Mice, Inbred Strains
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Hemocyanins
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Warren, R. W., Caster, S. A., Roths, J. B., Murphy, E. D., & Pisetsky, D. S. (1984). The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains. Clin Immunol Immunopathol, 31(1), 65–77. https://doi.org/10.1016/0090-1229(84)90190-9
Warren, R. W., S. A. Caster, J. B. Roths, E. D. Murphy, and D. S. Pisetsky. “The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains.Clin Immunol Immunopathol 31, no. 1 (April 1984): 65–77. https://doi.org/10.1016/0090-1229(84)90190-9.
Warren RW, Caster SA, Roths JB, Murphy ED, Pisetsky DS. The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains. Clin Immunol Immunopathol. 1984 Apr;31(1):65–77.
Warren, R. W., et al. “The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains.Clin Immunol Immunopathol, vol. 31, no. 1, Apr. 1984, pp. 65–77. Pubmed, doi:10.1016/0090-1229(84)90190-9.
Warren RW, Caster SA, Roths JB, Murphy ED, Pisetsky DS. The influence of the lpr gene on B cell activation: differential antibody expression in lpr congenic mouse strains. Clin Immunol Immunopathol. 1984 Apr;31(1):65–77.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1984

Volume

31

Issue

1

Start / End Page

65 / 77

Location

United States

Related Subject Headings

  • Rheumatoid Factor
  • Mice, Inbred Strains
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Hemocyanins
  • Female