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Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice.

Publication ,  Journal Article
Pisetsky, DS; Caster, SA; Piper, M; Scott, DW; Steinberg, AD
Published in: Cell Immunol
March 1984

The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

March 1984

Volume

84

Issue

1

Start / End Page

32 / 40

Location

Netherlands

Related Subject Headings

  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred CBA
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Immunologic Deficiency Syndromes
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pisetsky, D. S., Caster, S. A., Piper, M., Scott, D. W., & Steinberg, A. D. (1984). Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice. Cell Immunol, 84(1), 32–40. https://doi.org/10.1016/0008-8749(84)90074-1
Pisetsky, D. S., S. A. Caster, M. Piper, D. W. Scott, and A. D. Steinberg. “Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice.Cell Immunol 84, no. 1 (March 1984): 32–40. https://doi.org/10.1016/0008-8749(84)90074-1.
Pisetsky DS, Caster SA, Piper M, Scott DW, Steinberg AD. Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice. Cell Immunol. 1984 Mar;84(1):32–40.
Pisetsky, D. S., et al. “Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice.Cell Immunol, vol. 84, no. 1, Mar. 1984, pp. 32–40. Pubmed, doi:10.1016/0008-8749(84)90074-1.
Pisetsky DS, Caster SA, Piper M, Scott DW, Steinberg AD. Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice. Cell Immunol. 1984 Mar;84(1):32–40.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

March 1984

Volume

84

Issue

1

Start / End Page

32 / 40

Location

Netherlands

Related Subject Headings

  • Spleen
  • Mice, Mutant Strains
  • Mice, Inbred CBA
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
  • Immunologic Deficiency Syndromes
  • Female