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Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA.

Publication ,  Journal Article
Jiang, W; Reich III, CF; Pisetsky, DS
Published in: Cell Immunol
May 2004

Bacterial DNA can stimulate the production of cytokines and nitric oxide (NO), while mammalian DNA can block these responses. If mammalian DNA is transfected into macrophages, however, it can stimulate NO production, without inducing IL-12. To define further this activity, signaling pathways induced by transfected calf thymus (CT) DNA were studied. Using RAW264.7 cells as a model, CT DNA in the transfection agent FuGENE 6 activated cells through the NF-kappaB and MAPKs pathways, similar to bacterial DNA and LPS. The role of these pathways was further investigated using specific inhibitors, with studies indicating that NO production is blocked by inhibitors of NF-kappaB and p38 but not other MAPKs. These data indicate that the immune activity of DNA is influenced by context or intracellular location and that, when transfected into cells, mammalian DNA can activate cells through signaling pathways similar to those of bacterial DNA.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

May 2004

Volume

229

Issue

1

Start / End Page

31 / 40

Location

Netherlands

Related Subject Headings

  • Transfection
  • Pyridines
  • Nitric Oxide
  • NF-kappa B
  • Mitogen-Activated Protein Kinases
  • Mice
  • Macrophages
  • Macrophage Activation
  • Lipids
  • Lipid Metabolism
 

Citation

APA
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ICMJE
MLA
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Jiang, W., Reich III, C. F., & Pisetsky, D. S. (2004). Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA. Cell Immunol, 229(1), 31–40. https://doi.org/10.1016/j.cellimm.2004.06.003
Jiang, Weiwen, Charles F. Reich III, and David S. Pisetsky. “Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA.Cell Immunol 229, no. 1 (May 2004): 31–40. https://doi.org/10.1016/j.cellimm.2004.06.003.
Jiang W, Reich III CF, Pisetsky DS. Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA. Cell Immunol. 2004 May;229(1):31–40.
Jiang, Weiwen, et al. “Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA.Cell Immunol, vol. 229, no. 1, May 2004, pp. 31–40. Pubmed, doi:10.1016/j.cellimm.2004.06.003.
Jiang W, Reich III CF, Pisetsky DS. Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNA. Cell Immunol. 2004 May;229(1):31–40.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

May 2004

Volume

229

Issue

1

Start / End Page

31 / 40

Location

Netherlands

Related Subject Headings

  • Transfection
  • Pyridines
  • Nitric Oxide
  • NF-kappa B
  • Mitogen-Activated Protein Kinases
  • Mice
  • Macrophages
  • Macrophage Activation
  • Lipids
  • Lipid Metabolism