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CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity.

Publication ,  Journal Article
Sato, S; Ono, N; Steeber, DA; Pisetsky, DS; Tedder, TF
Published in: J Immunol
November 15, 1996

CD19 serves as a cell surface response regulator that establishes signaling thresholds critical for B lymphocyte development and activation. B lymphocytes from CD19-deficient mice are hyporesponsive to transmembrane signals, while B lymphocytes from mice that overexpress CD19 to even a small extent (25% increase) become hyperresponsive. The B-1 subpopulation of B lymphocytes is particularly sensitive to CD19 regulation, since their development is severely decreased in CD19-deficient mice. The effect of altered CD19 expression levels on the development of B cells was therefore examined using transgenic mice that express varying levels of cell surface CD19. In this study, immature B cells within the bone marrow of wild-type mice were found to specifically up-regulate CD19 expression levels by twofold as they mature, while CD5+ B cells within the spleen and peritoneum expressed even higher levels of CD19. The development of CD5+ B cells was severely decreased in CD19-deficient mice, while there was a linear correlation between increased CD19 expression levels and the increased frequency of CD5+ B cells within the peritoneum and spleen. In fact, CD5+ B cells became a major B lymphocyte population in mice that overexpressed CD19. Increased expression of CD19 also correlated with increased levels of endogenous anti-DNA Abs and rheumatoid factor. These results indicate that up-regulated expression of CD19 is functionally important for B cell development and that CD19 establishes signaling thresholds that regulate the generation of B-1 lymphocytes as well as the development of autoantibodies.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 15, 1996

Volume

157

Issue

10

Start / End Page

4371 / 4378

Location

United States

Related Subject Headings

  • Signal Transduction
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice, Inbred NZB
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Cell Differentiation
  • B-Lymphocyte Subsets
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sato, S., Ono, N., Steeber, D. A., Pisetsky, D. S., & Tedder, T. F. (1996). CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol, 157(10), 4371–4378.
Sato, S., N. Ono, D. A. Steeber, D. S. Pisetsky, and T. F. Tedder. “CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity.J Immunol 157, no. 10 (November 15, 1996): 4371–78.
Sato S, Ono N, Steeber DA, Pisetsky DS, Tedder TF. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996 Nov 15;157(10):4371–8.
Sato, S., et al. “CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity.J Immunol, vol. 157, no. 10, Nov. 1996, pp. 4371–78.
Sato S, Ono N, Steeber DA, Pisetsky DS, Tedder TF. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996 Nov 15;157(10):4371–4378.

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 15, 1996

Volume

157

Issue

10

Start / End Page

4371 / 4378

Location

United States

Related Subject Headings

  • Signal Transduction
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice, Inbred NZB
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Cell Differentiation
  • B-Lymphocyte Subsets