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Effect os xid on anti-DNA B-cell precursors.

Publication ,  Journal Article
Pisetsky, DS; Caster, SA; Steinberg, AD
Published in: Cell Immunol
June 1983

The influence of the xid gene on murine autoimmunity was investigated by precursor frequency analysis of anti-DNA-producing B cells in non-xid and congenic xid autoimmune and normal mice. Antibody responses were induced in vitro by lipopolysaccharides under limiting-dilution conditions with IgM anti-DNA and antitrinitrophenyl (TNP) determined as models of an autoantibody and nonautoantibody, respectively. All mice demonstrated high frequencies of precursors for anti-DNA without differences between the autoimmune (NZB and MRL-lpr/lpr) and nonautoimmune (CBA/J and DBA/2) strains. In these strains, the presence of the xid gene was associated with a marked reduction of anti-DNA. For NZB.xid mice, this loss of anti-DNA precursors was much greater than that for anti-TNP precursors, whose frequency was similar to that of NZB mice. In contrast, xid-bearing CBA/N, DBA/2.xid, and MRL.xid mice showed marked reductions in both anti-DNA and anti-TNP precursors relative to non-xid mice. The intact anti TNP response of NZB.xid mice may be an additional manifestation of immune abnormalities of the NZB strain and suggests that their B-cell populations differ from those of other mice in the conditions for activation. Since NZB.xid spleens had a preferential reduction of anti-DNA responses, this result suggests that in NZB mice precursors for anti-DNA antibodies are predominantly distributed in the B-cell subset affected by xid, explaining the abrogation of their autoantibody production. The equivalent number of precursors in non-xid mice indicates, however, that additional regulatory abnormalities underlie the change from precursor to autoantibody-producing B cell.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

June 1983

Volume

78

Issue

2

Start / End Page

326 / 332

Location

Netherlands

Related Subject Headings

  • X Chromosome
  • Trinitrobenzenes
  • Sex Chromosomes
  • Rats, Inbred Strains
  • Rats
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
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Pisetsky, D. S., Caster, S. A., & Steinberg, A. D. (1983). Effect os xid on anti-DNA B-cell precursors. Cell Immunol, 78(2), 326–332. https://doi.org/10.1016/0008-8749(83)90287-3
Pisetsky, D. S., S. A. Caster, and A. D. Steinberg. “Effect os xid on anti-DNA B-cell precursors.Cell Immunol 78, no. 2 (June 1983): 326–32. https://doi.org/10.1016/0008-8749(83)90287-3.
Pisetsky DS, Caster SA, Steinberg AD. Effect os xid on anti-DNA B-cell precursors. Cell Immunol. 1983 Jun;78(2):326–32.
Pisetsky, D. S., et al. “Effect os xid on anti-DNA B-cell precursors.Cell Immunol, vol. 78, no. 2, June 1983, pp. 326–32. Pubmed, doi:10.1016/0008-8749(83)90287-3.
Pisetsky DS, Caster SA, Steinberg AD. Effect os xid on anti-DNA B-cell precursors. Cell Immunol. 1983 Jun;78(2):326–332.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

June 1983

Volume

78

Issue

2

Start / End Page

326 / 332

Location

Netherlands

Related Subject Headings

  • X Chromosome
  • Trinitrobenzenes
  • Sex Chromosomes
  • Rats, Inbred Strains
  • Rats
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice
  • Male