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The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage.

Publication ,  Journal Article
Fermor, B; Weinberg, JB; Pisetsky, DS; Guilak, F
Published in: Osteoarthritis Cartilage
October 2005

OBJECTIVES: Articular cartilage is an avascular tissue that exists at low oxygen tension. Oxygen tension can influence the production of the pro-inflammatory mediators nitric oxide (NO) and prostaglandin E2 (PGE(2)) in cartilage, which are increased in osteoarthritis (OA). The synthesis of these molecules can be stimulated by mechanical stress, which is an important risk factor for OA. The objective of this study was to determine the influence of oxygen tension on the induction of NO and PGE(2) production in articular cartilage in response to mechanical stress. DESIGN: Intermittent mechanical compression (0.05MPa, 0.5Hz for 24h) was applied to full thickness skeletally mature porcine articular cartilage explants at either 20%, 5%, or 1% O(2). NO, PGE(2) and peroxynitrite formation were measured, and the effect of the selective nitric oxide synthase 2 inhibitor 1400W was tested. RESULTS: Incubating articular cartilage at 5% O(2) significantly increased (P<0.001) baseline NO production, as compared with 1% or 20% O(2). Peroxynitrite formation was lower at reduced oxygen tension. Mechanical compression significantly increased (P<0.001) NO production at 20% O(2) but not at 5% or 1% O(2), and significantly increased (P<0.001) PGE(2) production at 20% O(2) (50 fold) and 5% O(2) (4 fold) but not at 1% O(2). 1400W blocked mechanically induced NO production and further increased PGE(2) production at 5% O(2) (P<0.05). CONCLUSIONS: Oxygen tension influences the endogenous production of NO and PGE(2) in cartilage and can have a significant effect on the induction of these inflammatory mediators in response to mechanical compression.

Duke Scholars

Published In

Osteoarthritis Cartilage

DOI

ISSN

1063-4584

Publication Date

October 2005

Volume

13

Issue

10

Start / End Page

935 / 941

Location

England

Related Subject Headings

  • Tyrosine
  • Tissue Culture Techniques
  • Swine
  • Stress, Mechanical
  • Oxygen
  • Nitric Oxide
  • Inflammation Mediators
  • Female
  • Dinoprostone
  • Cartilage, Articular
 

Citation

APA
Chicago
ICMJE
MLA
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Fermor, B., Weinberg, J. B., Pisetsky, D. S., & Guilak, F. (2005). The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage. Osteoarthritis Cartilage, 13(10), 935–941. https://doi.org/10.1016/j.joca.2005.05.001
Fermor, Beverley, J Brice Weinberg, David S. Pisetsky, and Farshid Guilak. “The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage.Osteoarthritis Cartilage 13, no. 10 (October 2005): 935–41. https://doi.org/10.1016/j.joca.2005.05.001.
Fermor B, Weinberg JB, Pisetsky DS, Guilak F. The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage. Osteoarthritis Cartilage. 2005 Oct;13(10):935–41.
Fermor, Beverley, et al. “The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage.Osteoarthritis Cartilage, vol. 13, no. 10, Oct. 2005, pp. 935–41. Pubmed, doi:10.1016/j.joca.2005.05.001.
Fermor B, Weinberg JB, Pisetsky DS, Guilak F. The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage. Osteoarthritis Cartilage. 2005 Oct;13(10):935–941.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

ISSN

1063-4584

Publication Date

October 2005

Volume

13

Issue

10

Start / End Page

935 / 941

Location

England

Related Subject Headings

  • Tyrosine
  • Tissue Culture Techniques
  • Swine
  • Stress, Mechanical
  • Oxygen
  • Nitric Oxide
  • Inflammation Mediators
  • Female
  • Dinoprostone
  • Cartilage, Articular