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Immunostimulatory properties of genomic DNA from different bacterial species.

Publication ,  Journal Article
Neujahr, DC; Reich, CF; Pisetsky, DS
Published in: Immunobiology
February 1999

Bacterial DNA has potent immunological properties because of its content of immunostimulatory sequences centering on CpG motifs. To investigate whether DNA from various bacterial species differ in these properties, the activity of a panel of DNA was assessed in in vitro cultures of murine spleen cells. This panel varied in base composition and included DNA from Clostridium perfringens (CP), Escherichia coli (EC), Micrococcus lysodeikticus (MC), Staphylococcus aureus (SA), and, as a mammalian DNA control, calf thymus (CT) DNA. In assays of IL-12 and IFN-gamma production as well as B cell mitogenesis, these DNA showed marked differences in their immunostimulatory activity. For both cytokine and B cell responses, EC DNA demonstrated the highest activity while CP DNA had the lowest activity among the bacterial DNA. To determine whether differences in stimulatory capacity resulted from differences in cell uptake, the activity of DNA complexed with lipofectin was tested. While the addition of lipofectin to DNA increased stimulation by all DNA, it did not change the relative potency of the DNA tested. These results indicate that bacterial DNA differ in their immunostimulatory capacity, most likely reflecting their content of CpG motifs. These differences could affect the induction of innate immunity as well as the consequences of infection.

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Published In

Immunobiology

DOI

ISSN

0171-2985

Publication Date

February 1999

Volume

200

Issue

1

Start / End Page

106 / 119

Location

Netherlands

Related Subject Headings

  • Staphylococcus aureus
  • Spleen
  • Species Specificity
  • Phosphatidylethanolamines
  • Micrococcus
  • Mice
  • Lymphocyte Activation
  • Interleukin-12
  • Interferon-gamma
  • Immunology
 

Citation

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Neujahr, D. C., Reich, C. F., & Pisetsky, D. S. (1999). Immunostimulatory properties of genomic DNA from different bacterial species. Immunobiology, 200(1), 106–119. https://doi.org/10.1016/S0171-2985(99)80036-9
Neujahr, D. C., C. F. Reich, and D. S. Pisetsky. “Immunostimulatory properties of genomic DNA from different bacterial species.Immunobiology 200, no. 1 (February 1999): 106–19. https://doi.org/10.1016/S0171-2985(99)80036-9.
Neujahr DC, Reich CF, Pisetsky DS. Immunostimulatory properties of genomic DNA from different bacterial species. Immunobiology. 1999 Feb;200(1):106–19.
Neujahr, D. C., et al. “Immunostimulatory properties of genomic DNA from different bacterial species.Immunobiology, vol. 200, no. 1, Feb. 1999, pp. 106–19. Pubmed, doi:10.1016/S0171-2985(99)80036-9.
Neujahr DC, Reich CF, Pisetsky DS. Immunostimulatory properties of genomic DNA from different bacterial species. Immunobiology. 1999 Feb;200(1):106–119.
Journal cover image

Published In

Immunobiology

DOI

ISSN

0171-2985

Publication Date

February 1999

Volume

200

Issue

1

Start / End Page

106 / 119

Location

Netherlands

Related Subject Headings

  • Staphylococcus aureus
  • Spleen
  • Species Specificity
  • Phosphatidylethanolamines
  • Micrococcus
  • Mice
  • Lymphocyte Activation
  • Interleukin-12
  • Interferon-gamma
  • Immunology