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The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice.

Publication ,  Journal Article
Jones, FS; Pisetsky, DS; Kurlander, RJ
Published in: Clin Immunol Immunopathol
April 1986

To study the assembly of DNA-anti-DNA complexes in vivo, we have measured the clearance from blood and organ localization of a murine IgG2a monoclonal anti-DNA antibody, called 6/0, following the infusion of DNA intravenously or intraperitoneally. Intraperitoneal DNA caused a profound acceleration of 6/0 anti-DNA clearance that was dose dependent and demonstrable after the infusion of as little as 1.9 microgram per gram of body weight of single-stranded DNA. The antibody was cleared primarily in the liver without increased deposition in the kidney. Intraperitoneal infusions of DNA also accelerated the clearance of 6/0 in autoimmune MRL-lpr/lpr mice. In contrast, intravenous DNA given in comparable doses caused only a slight increase in 6/0 antibody clearance; this accelerated clearance was seen only at low antigen doses and only during the first 10 min following DNA infusion. Using double-radiolabeling techniques, 6/0 and Cl.18, an IgG2ak myeloma protein without anti-DNA activity, were found to disappear from blood at a comparable rate in both B6D2 mice and MRL-lpr/lpr mice. These results suggest that the DNA-anti-DNA immune complexes can form in vivo but that this process is profoundly affected by the manner in which DNA enters the circulation. In addition, the results suggest that DNA-dependent clearance is not a major pathway for anti-DNA metabolism in normal or at least one strain of autoimmune mice.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1986

Volume

39

Issue

1

Start / End Page

49 / 60

Location

United States

Related Subject Headings

  • Species Specificity
  • Mice, Inbred Strains
  • Mice
  • Metabolic Clearance Rate
  • Male
  • Kinetics
  • Iodine Radioisotopes
  • Infusions, Parenteral
  • Immunology
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
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Jones, F. S., Pisetsky, D. S., & Kurlander, R. J. (1986). The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice. Clin Immunol Immunopathol, 39(1), 49–60. https://doi.org/10.1016/0090-1229(86)90204-7
Jones, F. S., D. S. Pisetsky, and R. J. Kurlander. “The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice.Clin Immunol Immunopathol 39, no. 1 (April 1986): 49–60. https://doi.org/10.1016/0090-1229(86)90204-7.
Jones FS, Pisetsky DS, Kurlander RJ. The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice. Clin Immunol Immunopathol. 1986 Apr;39(1):49–60.
Jones, F. S., et al. “The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice.Clin Immunol Immunopathol, vol. 39, no. 1, Apr. 1986, pp. 49–60. Pubmed, doi:10.1016/0090-1229(86)90204-7.
Jones FS, Pisetsky DS, Kurlander RJ. The clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice. Clin Immunol Immunopathol. 1986 Apr;39(1):49–60.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1986

Volume

39

Issue

1

Start / End Page

49 / 60

Location

United States

Related Subject Headings

  • Species Specificity
  • Mice, Inbred Strains
  • Mice
  • Metabolic Clearance Rate
  • Male
  • Kinetics
  • Iodine Radioisotopes
  • Infusions, Parenteral
  • Immunology
  • Disease Models, Animal