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Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice.

Publication ,  Journal Article
Zhu, S; Malhotra, A; Zhang, L; Deng, S; Zhang, T; Freedman, NJ; Storms, R; Peppel, K; Goldschmidt-Clermont, PJ; Dong, C
Published in: Atherosclerosis
September 2010

AIMS: Vein graft endothelial damage is a key step in the development of neointimal hyperplasia, leading to vein graft failure. We sought to determine whether exogenous endothelial progenitor cells could promote vein graft re-endothelialization, and thereby ameliorate neointimal hyperplasia. METHODS AND RESULTS: Carotid artery interposition grafting was performed with syngeneic inferior vena cavae in mice with severe combined immunodeficiency (SCID). Lineage-negative human umbilical cord blood (hUCB) cells (or medium alone) were injected into vein-grafted mice intra-operatively and 2 weeks post-operatively. In vein grafts from hUCB cell-injected mice, we found human HLA-expressing endothelial cells, as well as increased levels of VEGF and FGF-2. Furthermore, hUCB cells secreted VEGF and FGF-2 in vitro. The markedly enhanced endothelial regeneration, likely resulting from both direct engraftment and paracrine actions of hUCB cells, inhibited inflammatory response, diminished intimal cell proliferation, and reduced neointimal hyperplasia in the vein grafts. CONCLUSIONS: hUCB cells may accelerate vein graft re-endothelialization via both direct differentiation into endothelial cells and release of paracrine factors to enhance endothelial regeneration and reduce inflammation. These data highlight a potential therapeutic role for cellular therapy in vessel injury.

Duke Scholars

Published In

Atherosclerosis

DOI

EISSN

1879-1484

Publication Date

September 2010

Volume

212

Issue

1

Start / End Page

63 / 69

Location

Ireland

Related Subject Headings

  • Vena Cava, Inferior
  • Vascular Endothelial Growth Factor A
  • Tunica Intima
  • Time Factors
  • Regeneration
  • Paracrine Communication
  • Mice, SCID
  • Mice, Inbred C57BL
  • Mice
  • Inflammation
 

Citation

APA
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ICMJE
MLA
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Zhu, S., Malhotra, A., Zhang, L., Deng, S., Zhang, T., Freedman, N. J., … Dong, C. (2010). Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice. Atherosclerosis, 212(1), 63–69. https://doi.org/10.1016/j.atherosclerosis.2010.04.018
Zhu, Shoukang, Anuj Malhotra, Lisheng Zhang, Shanming Deng, Taifang Zhang, Neil J. Freedman, Robert Storms, Karsten Peppel, Pascal J. Goldschmidt-Clermont, and Chunming Dong. “Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice.Atherosclerosis 212, no. 1 (September 2010): 63–69. https://doi.org/10.1016/j.atherosclerosis.2010.04.018.
Zhu S, Malhotra A, Zhang L, Deng S, Zhang T, Freedman NJ, et al. Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice. Atherosclerosis. 2010 Sep;212(1):63–9.
Zhu, Shoukang, et al. “Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice.Atherosclerosis, vol. 212, no. 1, Sept. 2010, pp. 63–69. Pubmed, doi:10.1016/j.atherosclerosis.2010.04.018.
Zhu S, Malhotra A, Zhang L, Deng S, Zhang T, Freedman NJ, Storms R, Peppel K, Goldschmidt-Clermont PJ, Dong C. Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice. Atherosclerosis. 2010 Sep;212(1):63–69.
Journal cover image

Published In

Atherosclerosis

DOI

EISSN

1879-1484

Publication Date

September 2010

Volume

212

Issue

1

Start / End Page

63 / 69

Location

Ireland

Related Subject Headings

  • Vena Cava, Inferior
  • Vascular Endothelial Growth Factor A
  • Tunica Intima
  • Time Factors
  • Regeneration
  • Paracrine Communication
  • Mice, SCID
  • Mice, Inbred C57BL
  • Mice
  • Inflammation