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CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis.

Publication ,  Journal Article
Sempowski, GD; Cross, SJ; Heinly, CS; Scearce, RM; Haynes, BF
Published in: J Immunol
January 15, 2004

CD7 and CD28 are T cell Ig superfamily molecules that share common signaling mechanisms. To determine roles CD7 and CD28 might play in peripheral lymphocyte development and function, we have generated CD7/CD28-double-deficient mice. CD7- and CD28-single-deficient and CD7/CD28-double-deficient mice had normal levels of CD4 and CD8-single-positive T cells in thymus and spleen. However, CD28-deficient mice had decreased CD4+CD25+ T cells in spleen compared with wild-type mice, and CD7/CD28-double-deficient mice had decreased numbers of CD4+CD25+ T cells in both thymus and spleen compared with both wild-type and CD28-deficient mice. Functional studies demonstrated that CD4+CD25+ T cells from CD28-deficient and CD7/CD28-double-deficient mice could mediate suppression of CD3 mAb activation of CD4+CD25- wild-type T cells, but were less potent than wild-type CD4+CD25+ T regulatory cells. Thyroiditis developed in aged CD7/CD28-double-deficient mice (>1 year) that was not seen in age-matched control mice or single CD7- or CD28-deficient mice, thus suggesting in vivo loss of T regulatory cells allowed for the development of spontaneous thyroiditis. Taken together, these data demonstrated collaborative roles for both CD7 and CD28 in determination of number and function of CD4+CD25+ T regulatory cells in the thymus and peripheral immune sites and in the development of spontaneous thyroiditis.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

January 15, 2004

Volume

172

Issue

2

Start / End Page

787 / 794

Location

United States

Related Subject Headings

  • Thyroiditis
  • Thymus Gland
  • T-Lymphocyte Subsets
  • Spleen
  • Receptors, Interleukin-2
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sempowski, G. D., Cross, S. J., Heinly, C. S., Scearce, R. M., & Haynes, B. F. (2004). CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis. J Immunol, 172(2), 787–794. https://doi.org/10.4049/jimmunol.172.2.787
Sempowski, Gregory D., Scott J. Cross, Craig S. Heinly, Richard M. Scearce, and Barton F. Haynes. “CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis.J Immunol 172, no. 2 (January 15, 2004): 787–94. https://doi.org/10.4049/jimmunol.172.2.787.
Sempowski GD, Cross SJ, Heinly CS, Scearce RM, Haynes BF. CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis. J Immunol. 2004 Jan 15;172(2):787–94.
Sempowski, Gregory D., et al. “CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis.J Immunol, vol. 172, no. 2, Jan. 2004, pp. 787–94. Pubmed, doi:10.4049/jimmunol.172.2.787.
Sempowski GD, Cross SJ, Heinly CS, Scearce RM, Haynes BF. CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis. J Immunol. 2004 Jan 15;172(2):787–794.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

January 15, 2004

Volume

172

Issue

2

Start / End Page

787 / 794

Location

United States

Related Subject Headings

  • Thyroiditis
  • Thymus Gland
  • T-Lymphocyte Subsets
  • Spleen
  • Receptors, Interleukin-2
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Male