Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men.

Publication ,  Journal Article
Li, H; Bar, KJ; Wang, S; Decker, JM; Chen, Y; Sun, C; Salazar-Gonzalez, JF; Salazar, MG; Learn, GH; Morgan, CJ; Schumacher, JE; Hraber, P ...
Published in: PLoS Pathog
May 13, 2010

Elucidating virus-host interactions responsible for HIV-1 transmission is important for advancing HIV-1 prevention strategies. To this end, single genome amplification (SGA) and sequencing of HIV-1 within the context of a model of random virus evolution has made possible for the first time an unambiguous identification of transmitted/founder viruses and a precise estimation of their numbers. Here, we applied this approach to HIV-1 env analyses in a cohort of acutely infected men who have sex with men (MSM) and found that a high proportion (10 of 28; 36%) had been productively infected by more than one virus. In subjects with multivariant transmission, the minimum number of transmitted viruses ranged from 2 to 10 with viral recombination leading to rapid and extensive genetic shuffling among virus lineages. A combined analysis of these results, together with recently published findings based on identical SGA methods in largely heterosexual (HSX) cohorts, revealed a significantly higher frequency of multivariant transmission in MSM than in HSX [19 of 50 subjects (38%) versus 34 of 175 subjects (19%); Fisher's exact p = 0.008]. To further evaluate the SGA strategy for identifying transmitted/founder viruses, we analyzed 239 overlapping 5' and 3' half genome or env-only sequences from plasma viral RNA (vRNA) and blood mononuclear cell DNA in an MSM subject who had a particularly well-documented virus exposure history 3-6 days before symptom onset and 14-17 days before peak plasma viremia (47,600,000 vRNA molecules/ml). All 239 sequences coalesced to a single transmitted/founder virus genome in a time frame consistent with the clinical history, and a molecular clone of this genome encoded replication competent virus in accord with model predictions. Higher multiplicity of HIV-1 infection in MSM compared with HSX is consistent with the demonstrably higher epidemiological risk of virus acquisition in MSM and could indicate a greater challenge for HIV-1 vaccines than previously recognized.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

May 13, 2010

Volume

6

Issue

5

Start / End Page

e1000890

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virulence
  • Virology
  • Sexual Behavior
  • Risk Factors
  • Recombination, Genetic
  • Models, Genetic
  • Male
  • Humans
  • Homosexuality, Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, H., Bar, K. J., Wang, S., Decker, J. M., Chen, Y., Sun, C., … Shaw, G. M. (2010). High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men. PLoS Pathog, 6(5), e1000890. https://doi.org/10.1371/journal.ppat.1000890
Li, Hui, Katharine J. Bar, Shuyi Wang, Julie M. Decker, Yalu Chen, Chuanxi Sun, Jesus F. Salazar-Gonzalez, et al. “High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men.PLoS Pathog 6, no. 5 (May 13, 2010): e1000890. https://doi.org/10.1371/journal.ppat.1000890.
Li H, Bar KJ, Wang S, Decker JM, Chen Y, Sun C, et al. High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men. PLoS Pathog. 2010 May 13;6(5):e1000890.
Li, Hui, et al. “High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men.PLoS Pathog, vol. 6, no. 5, May 2010, p. e1000890. Pubmed, doi:10.1371/journal.ppat.1000890.
Li H, Bar KJ, Wang S, Decker JM, Chen Y, Sun C, Salazar-Gonzalez JF, Salazar MG, Learn GH, Morgan CJ, Schumacher JE, Hraber P, Giorgi EE, Bhattacharya T, Korber BT, Perelson AS, Eron JJ, Cohen MS, Hicks CB, Haynes BF, Markowitz M, Keele BF, Hahn BH, Shaw GM. High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men. PLoS Pathog. 2010 May 13;6(5):e1000890.

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

May 13, 2010

Volume

6

Issue

5

Start / End Page

e1000890

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virulence
  • Virology
  • Sexual Behavior
  • Risk Factors
  • Recombination, Genetic
  • Models, Genetic
  • Male
  • Humans
  • Homosexuality, Male