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Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor.

Publication ,  Journal Article
Yu, J-S; Liao, H-X; Gerdon, AE; Huffman, B; Scearce, RM; McAdams, M; Alam, SM; Popernack, PM; Sullivan, NJ; Wright, D; Cliffel, DE; Nabel, GJ ...
Published in: J Virol Methods
November 2006

Ebola virus (EBOV) Zaire, Sudan, as well as Ivory Coast are virulent human EBOV species. Both polyclonal and monoclonal antibodies (MAbs) were developed against soluble EBOV envelope glycoprotein (GP) for the study of EBOV envelope diversity and development of diagnostic reagents. Three EBOV Sudan-Gulu GP peptides, from the N-terminus, mid-GP, and C-terminus regions were used to immunize rabbits for the generation of anti-EBOV polyclonal antibodies. Polyclonal antisera raised against the C-terminus peptide could detect both Sudan-Gulu as well as Zaire GPs, while anti-N and mid-region peptide polyclonal sera recognized only EBOV Sudan-Gulu GP. Of the three anti-EBOV GP mouse MAbs produced, MAb 15H10 recognized all human EBOV GP species tested (Zaire, Sudan and Ivory Coast), and as well as reacted with the Reston non-human primate EBOV GPs. In addition, MAb 15H10 bound virion-associated GP of all known EBOV species. MAb 17A3 recognized GPs of both EBOV Sudan-Gulu and Zaire, while MAb 6D11 recognized only EBOV Sudan-Gulu GP. To detect EBOV GP, these antibody reagents were used in ELISA, surface plasmon resonance and in a quartz crystal microbalance immunosensor. Thus, polyclonal and monoclonal antibodies can be used in combination to identify and differentiate both human and non-human primate EBOV GPs.

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Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

November 2006

Volume

137

Issue

2

Start / End Page

219 / 228

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Surface Plasmon Resonance
  • Rabbits
  • Mice, Inbred BALB C
  • Mice
  • Immunoassay
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay
 

Citation

APA
Chicago
ICMJE
MLA
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Yu, J.-S., Liao, H.-X., Gerdon, A. E., Huffman, B., Scearce, R. M., McAdams, M., … Haynes, B. F. (2006). Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. J Virol Methods, 137(2), 219–228. https://doi.org/10.1016/j.jviromet.2006.06.014
Yu, Jae-Sung, Hua-Xin Liao, Aren E. Gerdon, Brian Huffman, Richard M. Scearce, Mille McAdams, S Munir Alam, et al. “Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor.J Virol Methods 137, no. 2 (November 2006): 219–28. https://doi.org/10.1016/j.jviromet.2006.06.014.
Yu J-S, Liao H-X, Gerdon AE, Huffman B, Scearce RM, McAdams M, et al. Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. J Virol Methods. 2006 Nov;137(2):219–28.
Yu, Jae-Sung, et al. “Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor.J Virol Methods, vol. 137, no. 2, Nov. 2006, pp. 219–28. Pubmed, doi:10.1016/j.jviromet.2006.06.014.
Yu J-S, Liao H-X, Gerdon AE, Huffman B, Scearce RM, McAdams M, Alam SM, Popernack PM, Sullivan NJ, Wright D, Cliffel DE, Nabel GJ, Haynes BF. Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. J Virol Methods. 2006 Nov;137(2):219–228.
Journal cover image

Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

November 2006

Volume

137

Issue

2

Start / End Page

219 / 228

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Surface Plasmon Resonance
  • Rabbits
  • Mice, Inbred BALB C
  • Mice
  • Immunoassay
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay