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Morphometric analysis for early detection of changes in cellular structure in a toxicological experiment.

Publication ,  Journal Article
Crotty, S; Roy Choudhury, K
Published in: Stat Med
December 20, 2007

This paper presents a method for semi-automatic analysis of morphometry in image cytometry studies. We examine morphological changes in neuroblastoma cells caused by a toxin. Nuclei are automatically recognized from images using a constrained seeded region growing algorithm. The shape of the nuclei is quantified by fitting ellipses to the identified regions. Features such as centroids, elongation, orientation and size are extracted from this fitting process. Under certain assumptions, it is demonstrated that the log elongation measurement has an asymptotically normal distribution whose variance is dependent only on object size. This allows weighted linear models to be fitted to elongation measurements. Hypothesis tests show that over time, there is a significant elongation due to application of the toxin. The other variables are not significant, but there is a significant 'image' effect. The scientific implications of these findings are considered.

Duke Scholars

Published In

Stat Med

DOI

ISSN

0277-6715

Publication Date

December 20, 2007

Volume

26

Issue

29

Start / End Page

5253 / 5266

Location

England

Related Subject Headings

  • Subtraction Technique
  • Statistics & Probability
  • Pattern Recognition, Automated
  • Neurotoxins
  • Neuroblastoma
  • Linear Models
  • Laser Scanning Cytometry
  • Image Enhancement
  • Humans
  • Cell Nucleus Size
 

Citation

APA
Chicago
ICMJE
MLA
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Crotty, S., & Roy Choudhury, K. (2007). Morphometric analysis for early detection of changes in cellular structure in a toxicological experiment. Stat Med, 26(29), 5253–5266. https://doi.org/10.1002/sim.2917
Crotty, Suzanne, and Kingshuk Roy Choudhury. “Morphometric analysis for early detection of changes in cellular structure in a toxicological experiment.Stat Med 26, no. 29 (December 20, 2007): 5253–66. https://doi.org/10.1002/sim.2917.
Crotty, Suzanne, and Kingshuk Roy Choudhury. “Morphometric analysis for early detection of changes in cellular structure in a toxicological experiment.Stat Med, vol. 26, no. 29, Dec. 2007, pp. 5253–66. Pubmed, doi:10.1002/sim.2917.
Journal cover image

Published In

Stat Med

DOI

ISSN

0277-6715

Publication Date

December 20, 2007

Volume

26

Issue

29

Start / End Page

5253 / 5266

Location

England

Related Subject Headings

  • Subtraction Technique
  • Statistics & Probability
  • Pattern Recognition, Automated
  • Neurotoxins
  • Neuroblastoma
  • Linear Models
  • Laser Scanning Cytometry
  • Image Enhancement
  • Humans
  • Cell Nucleus Size