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Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.

Publication ,  Journal Article
Osawa, M; Erickson, HP
Published in: Methods Enzymol
2009

We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems.

Duke Scholars

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2009

Volume

464

Start / End Page

3 / 17

Location

United States

Related Subject Headings

  • Permeability
  • Molecular Biology
  • Membrane Proteins
  • Liposomes
  • Cytoskeletal Proteins
  • Biochemistry & Molecular Biology
  • Bacterial Proteins
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Osawa, M., & Erickson, H. P. (2009). Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings. Methods Enzymol, 464, 3–17. https://doi.org/10.1016/S0076-6879(09)64001-5
Osawa, Masaki, and Harold P. Erickson. “Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.Methods Enzymol 464 (2009): 3–17. https://doi.org/10.1016/S0076-6879(09)64001-5.
Osawa, Masaki, and Harold P. Erickson. “Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.Methods Enzymol, vol. 464, 2009, pp. 3–17. Pubmed, doi:10.1016/S0076-6879(09)64001-5.

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2009

Volume

464

Start / End Page

3 / 17

Location

United States

Related Subject Headings

  • Permeability
  • Molecular Biology
  • Membrane Proteins
  • Liposomes
  • Cytoskeletal Proteins
  • Biochemistry & Molecular Biology
  • Bacterial Proteins
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology