Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Publication ,  Journal Article
Guo, Z; Li, H; Han, M; Xu, T; Wu, X; Zhuang, Y
Published in: Immunol Lett
March 30, 2011

The Id3 gene has been shown to play important roles in the development and function of broad tissue types including B and T cells. Id3 deficient mice develop autoimmune disease similar to human Sjögren's syndrome. Both B and T lymphocytes have been implicated to contribute to the disease phenotype in this disease model. In order to gain a better understanding of individual cell types in this disease model, we generated an Id3 conditional allele. An LckCre transgene was used to induce Id3 deletion in developing T cells. We showed that the Id3 gene was efficiently disrupted in early thymocyte development prior to T cell receptor (TCR)-mediated positive selection. Consequently, thymocyte maturation was impaired in the conditional knockout mice. These mice developed exocrinopathy starting at two months of age and subsequently exhibited high incidence of lymphocyte infiltration to salivary glands between eight and 12 months of age. This progressive feature of disease development is very similar to those observed in Id3 germline knockout mice. This study establishes a new model for investigating the relationship between T cell development and autoimmune disease. Our observation provides an experimental case that autoimmune disease may be induced by acquired mutation in developing T cells.

Duke Scholars

Published In

Immunol Lett

DOI

EISSN

1879-0542

Publication Date

March 30, 2011

Volume

135

Issue

1-2

Start / End Page

34 / 42

Location

Netherlands

Related Subject Headings

  • Time Factors
  • T-Lymphocytes
  • Sjogren's Syndrome
  • Receptors, Antigen, T-Cell
  • Mice
  • Inhibitor of Differentiation Proteins
  • Immunology
  • Humans
  • Gene Deletion
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Guo, Z., Li, H., Han, M., Xu, T., Wu, X., & Zhuang, Y. (2011). Modeling Sjögren's syndrome with Id3 conditional knockout mice. Immunol Lett, 135(1–2), 34–42. https://doi.org/10.1016/j.imlet.2010.09.009
Guo, Zengli, Hongmei Li, Min Han, Tian Xu, Xiaohui Wu, and Yuan Zhuang. “Modeling Sjögren's syndrome with Id3 conditional knockout mice.Immunol Lett 135, no. 1–2 (March 30, 2011): 34–42. https://doi.org/10.1016/j.imlet.2010.09.009.
Guo Z, Li H, Han M, Xu T, Wu X, Zhuang Y. Modeling Sjögren's syndrome with Id3 conditional knockout mice. Immunol Lett. 2011 Mar 30;135(1–2):34–42.
Guo, Zengli, et al. “Modeling Sjögren's syndrome with Id3 conditional knockout mice.Immunol Lett, vol. 135, no. 1–2, Mar. 2011, pp. 34–42. Pubmed, doi:10.1016/j.imlet.2010.09.009.
Guo Z, Li H, Han M, Xu T, Wu X, Zhuang Y. Modeling Sjögren's syndrome with Id3 conditional knockout mice. Immunol Lett. 2011 Mar 30;135(1–2):34–42.
Journal cover image

Published In

Immunol Lett

DOI

EISSN

1879-0542

Publication Date

March 30, 2011

Volume

135

Issue

1-2

Start / End Page

34 / 42

Location

Netherlands

Related Subject Headings

  • Time Factors
  • T-Lymphocytes
  • Sjogren's Syndrome
  • Receptors, Antigen, T-Cell
  • Mice
  • Inhibitor of Differentiation Proteins
  • Immunology
  • Humans
  • Gene Deletion
  • Disease Models, Animal