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KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development.

Publication ,  Journal Article
Yu, J; Lei, K; Zhou, M; Craft, CM; Xu, G; Xu, T; Zhuang, Y; Xu, R; Han, M
Published in: Hum Mol Genet
March 15, 2011

Nuclear movement relative to cell bodies is a fundamental process during certain aspects of mammalian retinal development. During the generation of photoreceptor cells in the cell division cycle, the nuclei of progenitors oscillate between the apical and basal surfaces of the neuroblastic layer (NBL). This process is termed interkinetic nuclear migration (INM). Furthermore, newly formed photoreceptor cells migrate and form the outer nuclear layer (ONL). In the current study, we demonstrated that a KASH domain-containing protein, Syne-2/Nesprin-2, as well as SUN domain-containing proteins, SUN1 and SUN2, play critical roles during INM and photoreceptor cell migration in the mouse retina. A deletion mutation of Syne-2/Nesprin-2 or double mutations of Sun1 and Sun2 caused severe reduction of the thickness of the ONL, mislocalization of photoreceptor nuclei and profound electrophysiological dysfunction of the retina characterized by a reduction of a- and b-wave amplitudes. We also provide evidence that Syne-2/Nesprin-2 forms complexes with either SUN1 or SUN2 at the nuclear envelope to connect the nucleus with dynein/dynactin and kinesin molecular motors during the nuclear migrations in the retina. These key retinal developmental signaling results will advance our understanding of the mechanism of nuclear migration in the mammalian retina.

Duke Scholars

Published In

Hum Mol Genet

DOI

EISSN

1460-2083

Publication Date

March 15, 2011

Volume

20

Issue

6

Start / End Page

1061 / 1073

Location

England

Related Subject Headings

  • Telomere-Binding Proteins
  • Retina
  • Protein Structure, Tertiary
  • Photoreceptor Cells
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

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Chicago
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MLA
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Yu, J., Lei, K., Zhou, M., Craft, C. M., Xu, G., Xu, T., … Han, M. (2011). KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development. Hum Mol Genet, 20(6), 1061–1073. https://doi.org/10.1093/hmg/ddq549
Yu, Juehua, Kai Lei, Min Zhou, Cheryl M. Craft, Gezhi Xu, Tian Xu, Yuan Zhuang, Rener Xu, and Min Han. “KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development.Hum Mol Genet 20, no. 6 (March 15, 2011): 1061–73. https://doi.org/10.1093/hmg/ddq549.
Yu J, Lei K, Zhou M, Craft CM, Xu G, Xu T, et al. KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development. Hum Mol Genet. 2011 Mar 15;20(6):1061–73.
Yu, Juehua, et al. “KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development.Hum Mol Genet, vol. 20, no. 6, Mar. 2011, pp. 1061–73. Pubmed, doi:10.1093/hmg/ddq549.
Yu J, Lei K, Zhou M, Craft CM, Xu G, Xu T, Zhuang Y, Xu R, Han M. KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development. Hum Mol Genet. 2011 Mar 15;20(6):1061–1073.
Journal cover image

Published In

Hum Mol Genet

DOI

EISSN

1460-2083

Publication Date

March 15, 2011

Volume

20

Issue

6

Start / End Page

1061 / 1073

Location

England

Related Subject Headings

  • Telomere-Binding Proteins
  • Retina
  • Protein Structure, Tertiary
  • Photoreceptor Cells
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice