Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.
Publication
, Journal Article
Singer, KH; Scearce, RM; Tuck, DT; Whichard, LP; Denning, SM; Haynes, BF
Published in: J Invest Dermatol
February 1989
A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands of Mr 43,000 and 72-80,000. We used the 1B10 antibody with complement to eliminate most 1B10 positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us to grow highly enriched populations of human TE cells.
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Published In
J Invest Dermatol
DOI
ISSN
0022-202X
Publication Date
February 1989
Volume
92
Issue
2
Start / End Page
166 / 170
Location
United States
Related Subject Headings
- Thymus Gland
- Skin
- Monocytes
- Macrophages
- Humans
- Fibroblasts
- Epithelium
- Epithelial Cells
- Dermatology & Venereal Diseases
- Cytotoxicity, Immunologic
Citation
APA
Chicago
ICMJE
MLA
NLM
Singer, K. H., Scearce, R. M., Tuck, D. T., Whichard, L. P., Denning, S. M., & Haynes, B. F. (1989). Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages. J Invest Dermatol, 92(2), 166–170. https://doi.org/10.1111/1523-1747.ep12276685
Singer, K. H., R. M. Scearce, D. T. Tuck, L. P. Whichard, S. M. Denning, and B. F. Haynes. “Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.” J Invest Dermatol 92, no. 2 (February 1989): 166–70. https://doi.org/10.1111/1523-1747.ep12276685.
Singer KH, Scearce RM, Tuck DT, Whichard LP, Denning SM, Haynes BF. Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages. J Invest Dermatol. 1989 Feb;92(2):166–70.
Singer, K. H., et al. “Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.” J Invest Dermatol, vol. 92, no. 2, Feb. 1989, pp. 166–70. Pubmed, doi:10.1111/1523-1747.ep12276685.
Singer KH, Scearce RM, Tuck DT, Whichard LP, Denning SM, Haynes BF. Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages. J Invest Dermatol. 1989 Feb;92(2):166–170.
Published In
J Invest Dermatol
DOI
ISSN
0022-202X
Publication Date
February 1989
Volume
92
Issue
2
Start / End Page
166 / 170
Location
United States
Related Subject Headings
- Thymus Gland
- Skin
- Monocytes
- Macrophages
- Humans
- Fibroblasts
- Epithelium
- Epithelial Cells
- Dermatology & Venereal Diseases
- Cytotoxicity, Immunologic