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Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development.

Publication ,  Journal Article
Haynes, BF; Singer, KH; Denning, SM; Martin, ME
Published in: J Immunol
December 1, 1988

To define early stages of T cell maturation during human fetal thymic development, we have used mAb reactive with CD2, CD3, and TCR molecules in indirect immunofluorescence assays on a series of early human fetal thymic specimens. Using a technique of quantitating the relative proportions of fluorescent-positive cells present in tissue sections, we found at 8.5 wk of gestational age after arrival of CD7+ T cell precursors into the thymic rudiment, 60% of thymic CD7+ cells were CD2+, 4% were CD3+ and none was TCR-delta+ or TCR beta+. Moreover, cells reactive with anti-CD2 antibodies against T11(2) and T11(3) epitopes of CD2 as well as thymic stromal cells expressing the CD2 ligand, lymphocyte function associated Ag-3, were also present at 8.5 wk. From 9.5 wk to birth TCR beta+ cells increased to include greater than 90% of all CD7+ cells while TCR-delta+ cells fell from a peak of 11% of CD7+ cells at 9.5 wk to 1% of CD7+ cells at birth. These data suggest that epitopes of CD2 molecules are expressed early on during fetal thymic development. Moreover, these data suggest that CD7+, CD2+, cytoplasmic CD3+ T cell precursors in man give rise to both TCR-delta+ T cells as well as to T cells expressing TCR-alpha beta.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1, 1988

Volume

141

Issue

11

Start / End Page

3776 / 3784

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Receptors, Immunologic
  • Receptors, Antigen, T-Cell
  • Phenotype
  • Immunology
  • Humans
  • Gestational Age
  • Embryonic and Fetal Development
  • CD3 Complex
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Haynes, B. F., Singer, K. H., Denning, S. M., & Martin, M. E. (1988). Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development. J Immunol, 141(11), 3776–3784.
Haynes, B. F., K. H. Singer, S. M. Denning, and M. E. Martin. “Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development.J Immunol 141, no. 11 (December 1, 1988): 3776–84.
Haynes BF, Singer KH, Denning SM, Martin ME. Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development. J Immunol. 1988 Dec 1;141(11):3776–84.
Haynes, B. F., et al. “Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development.J Immunol, vol. 141, no. 11, Dec. 1988, pp. 3776–84.
Haynes BF, Singer KH, Denning SM, Martin ME. Analysis of expression of CD2, CD3, and T cell antigen receptor molecules during early human fetal thymic development. J Immunol. 1988 Dec 1;141(11):3776–3784.

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1, 1988

Volume

141

Issue

11

Start / End Page

3776 / 3784

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Receptors, Immunologic
  • Receptors, Antigen, T-Cell
  • Phenotype
  • Immunology
  • Humans
  • Gestational Age
  • Embryonic and Fetal Development
  • CD3 Complex