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Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans.

Publication ,  Journal Article
Haynes, BF; Mann, DL; Hemler, ME; Schroer, JA; Shelhamer, JH; Eisenbarth, GS; Strominger, JL; Thomas, CA; Mostowski, HS; Fauci, AS
Published in: Proc Natl Acad Sci U S A
May 1980

This study characterizes a monoclonal antibody (3A1), and partially characterizes the cell surface antigen and the functional peripheral blood T cell subset that it defines. The 3A1 antigen is present on the surface of several human T cell lines (HSB-2, CEM, MOLT-4, and others) in various amounts but is absent from the T cell line YT4E and all human B cell lines tested. Immunoprecipitation of an HSB-2 extract with 3A1 yielded one specific band with a molecular weight of approximately 40,000 in the presence of reducing agent. With directly fluoresceinated 3A1 antibody, fluorescence-activated cell sorter analysis showed that 85% of peripheral blood E-rosette-positive T cells were positive for the 3A1 antigen. After E-rosette-positive cells had been separated into 3A1+ and 3A1- cell suspensions, the 3A1+ cells helped autologous peripheral blood B cell suspensions toward pokeweed mitogen-driven proliferation and intracytoplasmic Ig production, whereas 3A1-T cells did not. Further, addition of 3A1- cells from some but not all normal subjects to cocultures of 3A1+ cells and B cells actively suppressed intracytoplasmic Ig production. However, the 3A1+T cell subset could be activated by concanavalin A to maximally suppress B cell Ig synthesis in vitro. Thus, the 3A1 antibody defines a major functional subject of peripheral blood T cells and should provide a useful marker for the study of human T cell function.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1980

Volume

77

Issue

5

Start / End Page

2914 / 2918

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Rosette Formation
  • Myeloma Proteins
  • Membrane Proteins
  • Lymphocyte Cooperation
  • Isoantibodies
  • Humans
  • Epitopes
  • Clone Cells
  • Antigens, Surface
 

Citation

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Haynes, B. F., Mann, D. L., Hemler, M. E., Schroer, J. A., Shelhamer, J. H., Eisenbarth, G. S., … Fauci, A. S. (1980). Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc Natl Acad Sci U S A, 77(5), 2914–2918. https://doi.org/10.1073/pnas.77.5.2914
Haynes, B. F., D. L. Mann, M. E. Hemler, J. A. Schroer, J. H. Shelhamer, G. S. Eisenbarth, J. L. Strominger, C. A. Thomas, H. S. Mostowski, and A. S. Fauci. “Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans.Proc Natl Acad Sci U S A 77, no. 5 (May 1980): 2914–18. https://doi.org/10.1073/pnas.77.5.2914.
Haynes BF, Mann DL, Hemler ME, Schroer JA, Shelhamer JH, Eisenbarth GS, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2914–8.
Haynes, B. F., et al. “Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans.Proc Natl Acad Sci U S A, vol. 77, no. 5, May 1980, pp. 2914–18. Pubmed, doi:10.1073/pnas.77.5.2914.
Haynes BF, Mann DL, Hemler ME, Schroer JA, Shelhamer JH, Eisenbarth GS, Strominger JL, Thomas CA, Mostowski HS, Fauci AS. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2914–2918.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1980

Volume

77

Issue

5

Start / End Page

2914 / 2918

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Rosette Formation
  • Myeloma Proteins
  • Membrane Proteins
  • Lymphocyte Cooperation
  • Isoantibodies
  • Humans
  • Epitopes
  • Clone Cells
  • Antigens, Surface